体外由乙酸盐生成甲烷和甲基辅酶M：乙酰辅酶A是乙酸盐所需的中间活化形式的证据。

In vitro methane and methyl coenzyme M formation from acetate: evidence that acetyl-CoA is the required intermediate activated form of acetate.

作者信息

Grahame D A, Stadtman T C

出版信息

Biochem Biophys Res Commun. 1987 Aug 31;147(1):254-8. doi: 10.1016/s0006-291x(87)80114-6.

Abstract

Buffer-soluble extracts of acetate-grown Methanosarcina barkeri catalyzed methanogenesis from acetate in the presence of hydrogen and ATP. The rates of methane formation from either acetate plus ATP, or acetylphosphate without ATP added, were approximately doubled by the addition of coenzyme A (CoA). In vitro methyl group transfer from [2-14C]acetate to form [14CH3]methyl coenzyme M (14CH3S-CoM) was monitored by causing the accumulation of 14CH3S-CoM (14CH3-SCH2CH2SO3-) in the presence of 2-bromoethanesulfonate. The rate of 14CH3S-CoM formation was increased 2.5-fold by 0.2 mM CoA.

摘要

以乙酸盐为生长底物的巴氏甲烷八叠球菌的缓冲液可溶性提取物，在氢气和ATP存在的情况下催化乙酸盐生成甲烷。添加辅酶A（CoA）后，由乙酸盐加ATP或不添加ATP的乙酰磷酸生成甲烷的速率大约提高了一倍。通过在2-溴乙烷磺酸盐存在的情况下使14CH3S-CoM（14CH3-SCH2CH2SO3-）积累，监测体外[2-14C]乙酸盐的甲基转移以形成[14CH3]甲基辅酶M（14CH3S-CoM）。0.2 mM CoA使14CH3S-CoM的形成速率提高了2.5倍。