甲基辅酶M，巴氏甲烷八叠球菌在C1化合物产甲烷异化过程中的一种中间体。
Methyl-coenzyme M, an intermediate in methanogenic dissimilation of C1 compounds by Methanosarcina barkeri.
作者信息
Shapiro S, Wolfe R S
出版信息
J Bacteriol. 1980 Feb;141(2):728-34. doi: 10.1128/jb.141.2.728-734.1980.
Abstract
Extracts of Methanosarcina barkeri possess a specific methyltransferase that catalyzes the transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid. Over a fourfold range in added 2-mercaptoethanesulfonic acid, the formation of 2-(methylthio)ethanesulfonic acid exhibited a 1:1 ratio to 2-mercaptoethanesulfonic acid added. This reaction required adenosine 5'-triphosphate; a maximal ratio (mole/mole) of 85 methyl groups was transferred per adenosine 5'-triphosphate added. The methyltransferase was found in extracts of methanol-grown cells as well as in extracts of hydrogen-grown cells. In extracts of cells grown on either substrate, 2-(methylthio)ethanesulfonic acid was formed from added methanol or methylamine but not from acetate.
摘要
巴氏甲烷八叠球菌提取物含有一种特定的甲基转移酶，该酶催化甲醇的甲基基团转移至2-巯基乙烷磺酸。在添加的2-巯基乙烷磺酸四倍范围内，2-(甲硫基)乙烷磺酸的形成与添加的2-巯基乙烷磺酸呈现1:1的比例关系。此反应需要5'-三磷酸腺苷；每添加一分子5'-三磷酸腺苷，转移的甲基基团的最大比例（摩尔/摩尔）为85。在甲醇生长细胞的提取物以及氢气生长细胞的提取物中均发现了该甲基转移酶。在以任何一种底物生长的细胞提取物中，添加的甲醇或甲胺可形成2-(甲硫基)乙烷磺酸，但乙酸盐不能形成。
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