Institute of Plant Science and Resources (IPSR), Okayama University, Kurashiki 710-0046, Japan.
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan; Asian Satellite Campuses Institute, Nagoya University, Nagoya 464-8601, Japan.
Virology. 2019 Jul;533:125-136. doi: 10.1016/j.virol.2019.05.008. Epub 2019 May 23.
There is still limited information on the diversity of (-)ssRNA viruses that infect fungi. Here, we have discovered two novel (-)ssRNA mycoviruses in the shiitake mushroom (Lentinula edodes). The first virus has a monopartite RNA genome and relates to that of mymonaviruses (Mononegavirales), especially to Hubei rhabdo-like virus 4 from arthropods and thus designated as Lentinula edodes negative-strand RNA virus 1. The second virus has a putative bipartite RNA genome and is related to the recently discovered bipartite or tripartite phenui-like viruses (Bunyavirales) associated with plants and ticks, and designated as Lentinula edodes negative-strand RNA virus 2 (LeNSRV2). LeNSRV2 is likely the first segmented (-)ssRNA virus known to infect fungi. Its smaller RNA segment encodes a putative nucleocapsid and a plant MP-like protein using a potential ambisense coding strategy. These findings enhance our understanding of the diversity, evolution and spread of (-)ssRNA viruses in fungi.
关于感染真菌的（-）ssRNA 病毒的多样性，目前信息仍然有限。在这里，我们在香菇（Lentinula edodes）中发现了两种新型（-）ssRNA 真菌病毒。第一种病毒具有单链 RNA 基因组，与 mononegavirales 病毒（单负链 RNA 病毒目）有关，特别是与节肢动物的湖北杆状病毒 4 有关，因此被命名为香菇负链 RNA 病毒 1。第二种病毒具有假定的二分体 RNA 基因组，与最近发现的与植物和蜱相关的二分体或三分体 phenui 样病毒（布尼亚病毒目）有关，被命名为香菇负链 RNA 病毒 2（LeNSRV2）。LeNSRV2 可能是已知感染真菌的第一个分段（-）ssRNA 病毒。它较小的 RNA 片段使用潜在的双义编码策略编码一个假定的核衣壳蛋白和一个植物 MP 样蛋白。这些发现增进了我们对真菌中（-）ssRNA 病毒多样性、进化和传播的理解。
