Runnegar M T, Falconer I R, Silver J
Naunyn Schmiedebergs Arch Pharmacol. 1981 Nov;317(3):268-72. doi: 10.1007/BF00503829.
The effect of the peptide hepatotoxin from the bloom-forming blue-green alga Microcystis aeruginosa was investigated on isolated rat hepatocytes. When toxin was added to hepatocyte suspensions it produced deformation of the cells, as shown by scanning electron microscopy. This was apparent within 5 min of addition of toxin to the cells and the response was dose dependent: 30 ng of toxin was sufficient to cause deformation in 58 +/- 9% of 1.4 x 10(6) hepatocytes/ml of incubation. The deformation did not lead to cell death as measured by Trypan blue uptake within 120 min. Deoxycholate, cholate bromosulphophthalein, and rifampicin were found to prevent the deformation of hepatocytes by Microcystis aeruginosa toxin in a dose dependent manner, analogous to the effect of these agents on the response of hepatocytes to added phalloidin. This suggests that Microcystis aeruginosa toxin is transported into hepatocytes in the same way as phalloidin; namely sharing a transport system for bile acids on the hepatocyte plasma membrane.
研究了形成水华的蓝藻铜绿微囊藻产生的肽类肝毒素对分离的大鼠肝细胞的影响。当毒素添加到肝细胞悬液中时，扫描电子显微镜显示细胞出现变形。在向细胞中添加毒素后5分钟内这种变形就很明显，且反应呈剂量依赖性：30纳克毒素足以使每毫升孵育液中1.4×10⁶个肝细胞中的58±9%发生变形。在120分钟内通过台盼蓝摄取检测发现，这种变形并未导致细胞死亡。发现脱氧胆酸盐、胆酸盐、溴磺酚酞和利福平以剂量依赖性方式阻止铜绿微囊藻毒素引起的肝细胞变形，类似于这些试剂对肝细胞对添加鬼笔环肽反应的影响。这表明铜绿微囊藻毒素与鬼笔环肽以相同方式转运到肝细胞中；即共享肝细胞质膜上的胆汁酸转运系统。
