Karray S, Merle-Béral H, Vazquez A, Gerard J P, Debre P, Galanaud P
INSERM U 131, Clamart, France.
Blood. 1987 Oct;70(4):1105-10.
We studied the effects of B cell directed growth factors on B lymphocytes from 11 patients with chronic lymphocytic leukemia (B-CLL). B-CLL lymphocytes were costimulated with anti-mu antibody (Ab) and with three growth factor preparations: recombinant IL2, B cell growth factor (BCGF) (20 kiloDalton (kD) BCGF) and a high molecular weight BCGF (50 kD BCGF). IL2 was the more active factor (in six of 11 patients). The effect of IL2 was dependent on a costimulation with anti-mu Ab or occurred independently of anti-mu Ab, according to the patients. This pattern of reactivity did not correlate with the presence or absence of the IL2 receptor (IL2-R) molecule on fresh B-CLL lymphocytes. Five patients responded to the 20 kD BCGF. Although four of them were also strong responders to IL2, one strongly responded to the 20 kD BCGF and did not respond to IL2. Only one patient responded to the 50 kD BCGF. When an anti-IL2-R Ab was introduced into the culture, only the responsiveness to IL2 was abolished: thus both 20 kD and 50 kD BCGFs activate B-CLL lymphocytes independently of the IL2-R. These results show that several B cell directed growth factors can act independently to support the proliferation of B-CLL lymphocytes.
我们研究了B细胞定向生长因子对11例慢性淋巴细胞白血病（B-CLL）患者B淋巴细胞的影响。用抗μ抗体（Ab）和三种生长因子制剂对B-CLL淋巴细胞进行共刺激：重组白细胞介素2（IL2）、B细胞生长因子（BCGF）（20千道尔顿（kD）BCGF）和高分子量BCGF（50 kD BCGF）。IL2是更具活性的因子（11例患者中有6例）。根据患者情况，IL2的作用取决于与抗μ Ab的共刺激，或者独立于抗μ Ab发生。这种反应模式与新鲜B-CLL淋巴细胞上IL2受体（IL2-R）分子的存在与否无关。5例患者对20 kD BCGF有反应。其中4例对IL2也是强反应者，但有1例对20 kD BCGF有强烈反应，对IL2无反应。只有1例患者对50 kD BCGF有反应。当将抗IL2-R Ab引入培养物中时，仅对IL2的反应性被消除：因此20 kD和50 kD BCGF均独立于IL2-R激活B-CLL淋巴细胞。这些结果表明，几种B细胞定向生长因子可独立发挥作用，支持B-CLL淋巴细胞的增殖。
