Vazquez A, Auffredou M T, Gerard J P, Delfraissy J F, Galanaud P
Institut National de la Santé et de la Recherche Médicale U131, Clamart, France.
J Immunol. 1987 Oct 1;139(7):2344-8.
A high m.w. B cell growth factor (50,000 BCGF) prepared from lectin-activated human peripheral blood lymphocyte culture supernatants acts only on B cells preactivated by a first signal. This first signal can be delivered in vitro (with anti-mu antibody (Ab)) or in vivo. Upon costimulation with anti-mu Ab the 50,000 BCGF induces an early and transient proliferative response, whereas the response to interleukin 2 (IL-2) develops more progressively. To determine the respective targets of the 50,000 BCGF and of IL-2, B cells were activated with anti-mu Ab and separated according to the expression of the IL-2 receptor (cluster designation (CD)25 antigen). CD25+ B cells do not respond to the 50,000 BCGF and do not acquire this responsiveness after an additional culture with IL-2. CD25- B cells respond to the 50,000 BCGF and not to IL-2. However, when CD25- B cells are cultured for 3 days with the 50,000 BCGF they become responsive to IL-2. These results demonstrate a pathway of B cell activation based on the ordered and sequential action of anti-mu Ab, the 50,000 BCGF, and IL-2.
从凝集素激活的人外周血淋巴细胞培养上清液中制备的高分子量B细胞生长因子（50,000 BCGF）仅作用于由第一信号预激活的B细胞。该第一信号可在体外（用抗μ抗体（Ab））或体内传递。在用抗μ抗体共刺激时，50,000 BCGF诱导早期短暂的增殖反应，而对白细胞介素2（IL-2）的反应发展更为渐进。为了确定50,000 BCGF和IL-2各自的靶标，用抗μ抗体激活B细胞，并根据IL-2受体（簇分化（CD）25抗原）的表达进行分离。CD25 + B细胞对50,000 BCGF无反应，在用IL-2进行额外培养后也不会获得这种反应性。CD25 - B细胞对50,000 BCGF有反应，对IL-2无反应。然而，当CD25 - B细胞与50,000 BCGF培养3天时，它们变得对IL-2有反应。这些结果证明了基于抗μ抗体、50,000 BCGF和IL-2有序和相继作用的B细胞激活途径。
