制何首乌对 D-半乳糖致衰老模型小鼠肝组织内脂肪酸含量的影响。
Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose.
机构信息
Department of Biochemistry and Molecular Biology, Zunyi Medical University, Zunyi, Guizhou Province, China.
Department of Pharmaceutical Analysis, Zunyi Medical University, Zunyi, Guizhou Province, China.
出版信息
Lipids Health Dis. 2019 Jun 1;18(1):128. doi: 10.1186/s12944-019-1055-y.
BACKGROUND
Polygonum Multiflorum Thunb(PMT) has multiple biological effects, such as anti-inflammatory, lipid-lowering, anti-aging and so on. Therefore, D-galactose-induced aging mice were used to study the effect of PMT on fatty acid metabolism and its underlying mechanism.
METHODS
C57BL/6 male mice were randomly divided into normal group, aging model group, PMT intragastrical administration group (high, Medium, low); model group and PMT intragastrical administration group Daily intraperitoneal injection D-galactose 800 mg·ml·Kg to establish subacute aging model; PMT intragastrical administration group at the same time to intragastrical PMT extract (1 g·ml·Kg, 0.6 g·ml·Kg, 0.3 g·ml·Kg), normal group injection and intragastrical equivalent saline for 60 consecutive days. By detecting the oxidation index of liver to judge the efficacy of PMT, gas chromatography-mass spectrometry (GC-MS) analysis was used to quantitatively analyze the fatty acid content in liver.
RESULTS
Finally, we found that PMT improved the enzyme activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in aging mice, and reduce the enzyme activity of malondialdehyde (MDA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The content of fatty acids such as C18:1, C18:2, C18:3 N3, C20:2 and C20:3 N3 decreased significantly in senescent mice (P < 0.05) as evidenced by GC-MS analysis, whereas, these fatty acids increased significantly after treatment of PMT (P < 0.05).
CONCLUSION
PMT improves the content of liver fatty acids in aging mice induced by D-galactose through, enhancing the activity of anti-oxidant enzymes.
背景
何首乌（PMT）具有多种生物学作用，如抗炎、降血脂、抗衰老等。因此，本研究采用 D-半乳糖诱导衰老小鼠模型，探讨 PMT 对脂肪酸代谢的影响及其作用机制。
方法
雄性 C57BL/6 小鼠随机分为正常组、衰老模型组、PMT 灌胃给药组（高、中、低剂量组）；模型组和 PMT 灌胃给药组每日腹腔注射 D-半乳糖 800mg·ml·kg 建立亚急性衰老模型；PMT 灌胃给药组同时给予 PMT 灌胃提取液（1g·ml·kg、0.6g·ml·kg、0.3g·ml·kg），正常组注射并给予等体积生理盐水，连续 60 天。通过检测肝脏氧化指标判断 PMT 的药效，采用气相色谱-质谱联用（GC-MS）分析方法定量分析肝脏中脂肪酸的含量。
结果
结果发现，PMT 可提高衰老小鼠超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的酶活性，降低丙二醛（MDA）、天门冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）的酶活性。GC-MS 分析结果表明，衰老小鼠的 C18:1、C18:2、C18:3N3、C20:2 和 C20:3N3 等脂肪酸含量显著降低（P < 0.05），而 PMT 处理后这些脂肪酸含量显著增加（P < 0.05）。
结论
PMT 通过提高抗氧化酶的活性，改善 D-半乳糖诱导衰老小鼠肝脏脂肪酸含量。
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