Liu Y, Teng G G, Wang W H, Wu T, Hu F L
Department of Gastroenterology, Peking University First Hospital, Beijing 100034, China.
Zhonghua Yi Xue Za Zhi. 2019 May 28;99(20):1546-1552. doi: 10.3760/cma.j.issn.0376-2491.2019.20.006.
To investigate the effects of sucralfate suspension gel (SC) on () colonization, -induced gastric mucosal injury and gastrointestinal microecology in mice. C57BL/6J mice were randomly divided into 3 groups, including normal control (NC) group, model (HP) group, and SC prevention (HP+SC) group. infection mouse model was established by gavage with Sydney strain (SS1). And HP+SC group was also administered with SC for 14 days. After mice were sacrificed, the gastric mucosa was taken for HE staining, immunohistochemical (IHC) staining [, zonula occludens-1 (ZO-1), Occludin, interleukin (IL)-8, IL-10 and tumor necrosis factor-α (TNF-α)], qPCR (IL-8, IL-10, TNF-α) . And cell ultrastructure was observed by electron microscopy. Microbiota communities in the gastric mucosa or fecal were investigated using 16S ribosomal RNA (rRNA) gene sequencing. The detection of in IHC staining or Giemsa staining was defined as infection. The positive rate of HP group was 91.67%, and that of HP+SC group was 50.00%. The EDS of HP group were significantly higher than those of the NC group [(3.42±0.19) vs (1.17±0.17), 0.05], while EDS of HP+SC group (2.42±0.29) were significantly lower than that of HP group (0.05). The mRNA levels and immunostaining scores of IL-8, IL-10 and TNF-α in HP group was higher than NC group (all 0.05). Compared with HP group, mRNA levels and IHC scores of IL-8 significantly decreased (all 0.05) in HP+SC group, while those of IL-10 and TNF-α was similar between two groups (all 0.05). The IHC scores of ZO-1 and Occludin in HP group was significantly lower than NC group (all 0.05), and that of ZO-1 and Occludin in the HP+SC group increased compared with HP group [(2.00±0.26) vs (1.17±0.48), 0.05; (3.50±0.43) vs (2.33±0.21), 0.05]. Compared with NC group, the diversity of gastric microbiota in HP group was significantly lower, and diversity of gut microbiota decreased insignificantly. There was no significant difference between HP+SC group and HP group in gastric or gut microbiota. SC reduces colonization, protects -induced gastric mucosal injury, decreases -induced IL-8 expression, enhances Occludin. However, its effects on -induced gastrointestinal microbiota disorders are limited.
探讨硫糖铝混悬凝胶（SC）对小鼠幽门螺杆菌（Hp）定植、Hp诱导的胃黏膜损伤及胃肠道微生态的影响。将C57BL/6J小鼠随机分为3组，包括正常对照（NC）组、Hp模型（HP）组和SC预防（HP+SC）组。通过灌胃悉尼菌株（SS1）建立Hp感染小鼠模型。HP+SC组同时给予SC 14天。小鼠处死后，取胃黏膜进行苏木精-伊红（HE）染色、免疫组织化学（IHC）染色[细胞间黏附分子-1（ICAM-1）、紧密连接蛋白-1（ZO-1）、闭合蛋白（Occludin）、白细胞介素（IL）-8、IL-10和肿瘤坏死因子-α（TNF-α）]、实时荧光定量聚合酶链反应（qPCR）（IL-8、IL-10、TNF-α）检测。并通过电子显微镜观察细胞超微结构。采用16S核糖体RNA（rRNA）基因测序研究胃黏膜或粪便中的微生物群落。IHC染色或吉姆萨染色中Hp的检测定义为Hp感染。HP组的Hp阳性率为91.67%，HP+SC组为50.00%。HP组的内镜下十二指肠溃疡面积（EDS）显著高于NC组[（3.42±0.19）比（1.17±0.17），P<0.05]，而HP+SC组的EDS（2.42±0.29）显著低于HP组（P<0.05）。HP组中IL-8、IL-10和TNF-α的mRNA水平及免疫染色评分高于NC组（均P<0.05）。与HP组相比，HP+SC组中IL-8的mRNA水平和IHC评分显著降低（均P<0.05），而IL-10和TNF-α在两组间相似（均P>0.05）。HP组中ZO-1和Occludin的IHC评分显著低于NC组（均P<0.05），HP+SC组中ZO-1和Occludin的评分与HP组相比升高[（2.00±0.26）比（1.17±0.48），P<0.05；（3.50±0.43）比（2.33±0.21），P<0.05]。与NC组相比，HP组胃微生物群的多样性显著降低，肠道微生物群的多样性无明显降低。HP+SC组与HP组在胃或肠道微生物群方面无显著差异。SC减少Hp定植，保护Hp诱导的胃黏膜损伤，降低Hp诱导的IL-8表达，增强Occludin表达。然而，其对Hp诱导的胃肠道微生物群紊乱的影响有限。
