Dahyot Sandrine, Lebeurre Jérémie, Laumay Floriane, Argemi Xavier, Dubos Charline, Lemée Ludovic, Prévost Gilles, François Patrice, Pestel-Caron Martine
UNIROUEN, GRAM EA2656, Rouen University Hospital, Normandie Université, Rouen, France.
UNIROUEN, GRAM EA2656, Normandie Université, Rouen, France.
Front Microbiol. 2019 May 17;10:1109. doi: 10.3389/fmicb.2019.01109. eCollection 2019.
is increasingly recognized as a potent pathogen, responsible for severe infections with an outcome resembling that of . Here, we developed and evaluated a tool for typing, using DNA sequence analysis of the repeat-encoding region (R-domain) in the gene encoding the fibrinogen (Fg)-binding protein Fbl (-typing). We typed 240 isolates from various clinical and geographical origins. The length of the R-domain ranged from 9 to 52 repeats. -typing identified 54 unique 18-bp repeat sequences and 92 distinct -types. The discriminatory power of -typing was higher than that of multilocus sequence typing (MLST) and equivalent to that of tandem repeat sequence typing. -types could assign isolates to MLST clonal complexes with excellent predictive power. The ability to promote adherence to immobilized human Fg was evaluated for 55 isolates chosen to reflect the genetic diversity of the gene. We observed no direct correlation between Fg binding ability and -types. However, the lowest percentage of Fg binding was observed for isolates carrying a 5'-end frameshift mutation of the gene and for those harboring fewer than 43 repeats in the R-domain. qRT-PCR assays for some isolates revealed no correlation between gene expression and Fg binding capacity. In conclusion, this study shows that -typing is a useful tool in epidemiology, especially because it is an easy, cost-effective, rapid and portable method (http://fbl-typing.univ-rouen.fr/). The impact of polymorphism on the structure of the protein, its expression on the cell surface and in virulence remains to be determined.
越来越被认为是一种强效病原体，可导致严重感染，其结果与……相似。在此，我们开发并评估了一种用于……分型的工具，利用编码纤维蛋白原（Fg）结合蛋白Fbl的基因中重复编码区域（R结构域）的DNA序列分析（……分型）。我们对来自不同临床和地理来源的240株……分离株进行了分型。R结构域的长度范围为9至52个重复序列。……分型鉴定出54个独特的18碱基对重复序列和92种不同的……型。……分型的鉴别力高于多位点序列分型（MLST），与串联重复序列分型相当。……型能够以优异的预测能力将分离株归入MLST克隆复合体。对55株被选来反映……基因遗传多样性的分离株评估了其促进黏附固定化人Fg的能力。我们未观察到Fg结合能力与……型之间存在直接相关性。然而，在携带……基因5'端移码突变的分离株以及R结构域中重复序列少于43个的分离株中，观察到Fg结合的比例最低。对一些分离株进行的qRT-PCR分析显示……基因表达与Fg结合能力之间无相关性。总之，本研究表明……分型是……流行病学中的一种有用工具，特别是因为它是一种简便、经济高效、快速且便于携带的方法（http://fbl-typing.univ-rouen.fr/）。……多态性对蛋白质结构、其在细胞表面的表达以及毒力的影响仍有待确定。
