DprA对[具体物种]的自然感受态至关重要且具有保守的进化机制。

DprA Is Essential for Natural Competence in and Has a Conserved Evolutionary Mechanism.

作者信息

Huang Li, Tian Xiu, Liu Mafeng, Wang Mingshu, Biville Francis, Cheng Anchun, Zhu Dekang, Jia Renyong, Chen Shun, Zhao Xinxin, Yang Qiao, Wu Ying, Zhang Shaqiu, Huang Juan, Tian Bin, Yu Yanling, Liu Yunya, Zhang Ling, Pan Leichang, Rehman Mujeeb Ur, Chen Xiaoyue

机构信息

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

出版信息

Front Genet. 2019 May 17;10:429. doi: 10.3389/fgene.2019.00429. eCollection 2019.

ATCC11845 (RA ATCC11845) is naturally competent. However, the genes involved in natural transformation in this species remain largely unknown. Bioinformatic analysis predicts that DprA of RA (DprA) has three domains: a sterile alpha motif (SAM), a Rossmann fold (RF) domain and a Z-DNA-binding domain (Zα). Inactivation of abrogated natural transformation in RA ATCC11845, and this effect was restored by the expression of . The with SAM and RF domains of and the with RF and Zα domains of was able to restore natural transformation in the RA ATCC11845 mutant. An Arg123 mutation in the RF domain of was not able to restore natural transformation of the RA ATCC11845 mutant. Furthermore, DprA abolished its ability to bind DNA, suggesting that the RF domain is essential for the function of DprA. Finally, the of which has not been reported to be natural competent currently was partially able to restore natural transformation in RA ATCC11845 mutant. These results collectively suggest that DprA has a conserved evolutionary mechanism.

美国典型培养物保藏中心11845菌株（RA ATCC11845）具有天然感受态。然而，该物种中参与自然转化的基因在很大程度上仍不为人知。生物信息学分析预测，RA的DprA（DprA）有三个结构域：一个无活性α基序（SAM）、一个罗斯曼折叠（RF）结构域和一个Z-DNA结合结构域（Zα）。在RA ATCC11845中使[此处原文缺失相关基因名称]失活会消除自然转化，而通过[此处原文缺失相关基因名称]的表达可恢复这种效应。具有[此处原文缺失相关基因名称]的SAM和RF结构域的[此处原文缺失相关基因名称]以及具有[此处原文缺失相关基因名称]的RF和Zα结构域的[此处原文缺失相关基因名称]能够恢复RA ATCC11845[此处原文缺失相关基因名称]突变体的自然转化。[此处原文缺失相关基因名称]的RF结构域中的精氨酸123突变无法恢复RA ATCC11845[此处原文缺失相关基因名称]突变体的自然转化。此外，DprA丧失了其结合DNA的能力，这表明RF结构域对DprA的功能至关重要。最后，目前尚未报道具有天然感受态的[此处原文缺失相关物种名称]的[此处原文缺失相关基因名称]能够部分恢复RA ATCC11845[此处原文缺失相关基因名称]突变体的自然转化。这些结果共同表明DprA具有保守的进化机制。