Okubo T, Takahashi H, Sumitomo M, Shindoh K, Suzuki S
1st Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
Int Arch Allergy Appl Immunol. 1987;84(2):149-55. doi: 10.1159/000234414.
To study a role of leukotrienes (LTs) in bronchial asthma, we measured LTC4 and LTD4 in plasma of 16 asthmatics during wheezing attack and of 4 normal subjects. Plasma of arterial blood was processed by extraction using the SEP-PAK, followed by high-performance liquid chromatography (HPLC). Fractions from HPLC with elution times which corresponded to synthetic LTC4 and LTD4 were used for quantification of LTs by radioimmunoassay (RIA) and bioassay. LTC4 was detected in 13 out of 16 asthmatics by RIA (mean +/- SD, 410 +/- 630 pg/ml) and in 5 by bioassay. LTD4 was detected in 10 out of 16 asthmatics by RIA (mean +/- SD, 540 +/- 290 pg/ml) and in 7 by bioassay. No LTs were detected by either method in the normal subjects. The present study shows that LTC4 and LTD4 play a role as chemical mediators during asthmatic attack.
为研究白三烯（LTs）在支气管哮喘中的作用，我们检测了16例哮喘患者喘息发作时以及4例正常受试者血浆中的LTC4和LTD4。动脉血血浆通过SEP - PAK萃取处理，随后进行高效液相色谱法（HPLC）分析。HPLC洗脱时间与合成LTC4和LTD4相对应的馏分用于通过放射免疫测定法（RIA）和生物测定法对LTs进行定量分析。通过RIA在16例哮喘患者中的13例检测到LTC4（均值±标准差，410±630 pg/ml），通过生物测定法在5例中检测到。通过RIA在16例哮喘患者中的10例检测到LTD4（均值±标准差，540±290 pg/ml），通过生物测定法在7例中检测到。正常受试者中两种方法均未检测到LTs。本研究表明，LTC4和LTD4在哮喘发作期间作为化学介质发挥作用。
