Shiratsuki N, Uyama O, Kitada O, Suenaga N, Nakamura H, Sugita M, Hayashi Y, Yamamoto S
Fifth Department of Internal Medicine, Hyogo College of Medicine, Japan.
Prostaglandins Leukot Essent Fatty Acids. 1990 Aug;40(4):285-9. doi: 10.1016/0952-3278(90)90051-l.
To study the role of leukotriene C4(LTC4) and the effect of hydrocortisone and aminophylline on plasma LTC4 levels in patients with asthmatic attacks, we measured LTC4 in plasma of 18 asthmatics during a wheezing attack and of 7 normal subjects. Blood samples were obtained before and after treatment with aminophylline and/or hydrocortisone injections. We extracted LTC4 using a Sep-Pak C18 cartridge for the measurement of LTC4 by radioimmunoassay. The plasma levels of immunoreactive LTC4 (i-LTC4) of the normal subjects were 142 +/- 25 pg/ml (n = 7), while those of nonatopic type asthmatic patients with wheezing attacks were 208 +/- 68 pg/ml (n = 15) (p less than 0.01). Before and after treatment with both hydrocortisone succinate (100 mg) and aminophylline (250 mg), 6 asthmatic patients with wheezing attacks had a mean plasma level of i-LTC4 181 +/- 24 and 132 +/- 18 pg/ml (p less than 0.01), respectively. On the other hand, the treatment with aminophylline 250 mg alone increased the i-LTC4 levels from 178 +/- 19 pg/mg to 213 +/- 16 pg/mg (n = 6)(p less than 0.05), while treatment with hydrocortisone succinate 100 mg decreased the i-LTC4 level 0.05 from 284 +/- 99 pg/ml to 249 +/- 85 pg/ml (n = 4)(p less than 0.05). In conclusion, the present study shows that the i-LTC4 level in venous blood of patients with asthmatic attacks is decreased significantly by treatment with hydrocortisone succinate.
为研究白三烯C4（LTC4）的作用以及氢化可的松和氨茶碱对哮喘发作患者血浆LTC4水平的影响，我们测定了18例哮喘患者喘息发作时及7例正常受试者血浆中的LTC4。在注射氨茶碱和/或氢化可的松治疗前后采集血样。我们使用Sep-Pak C18柱提取LTC4，并用放射免疫分析法测定LTC4。正常受试者免疫反应性LTC4（i-LTC4）的血浆水平为142±25 pg/ml（n = 7），而有喘息发作的非特应性哮喘患者为208±68 pg/ml（n = 15）（p<0.01）。6例有喘息发作的哮喘患者在接受琥珀酸氢化可的松（100 mg）和氨茶碱（250 mg）联合治疗前后，i-LTC4的平均血浆水平分别为181±24和132±18 pg/ml（p<0.01）。另一方面，单独使用250 mg氨茶碱治疗使i-LTC4水平从178±19 pg/ml升高至213±16 pg/ml（n = 6）（p<0.05），而使用100 mg琥珀酸氢化可的松治疗使i-LTC4水平从284±99 pg/ml降至249±85 pg/ml（n = 4）（p<0.05）。总之，本研究表明，琥珀酸氢化可的松治疗可使哮喘发作患者静脉血中的i-LTC4水平显著降低。
