Isono T, Koshihara Y, Murota S, Fukuda Y, Furukawa S
Biochem Biophys Res Commun. 1985 Jul 16;130(1):486-92. doi: 10.1016/0006-291x(85)90443-7.
Peptide leukotriene (LT) such as LTC4, LTD4, LTE4 have been considered to be major mediators of immediate type hypersensitivity reaction such as asthma. We have developed a rapid and simple extraction method using a Sep-Pak C18 cartridge for the measurement of LTC4 by radioimmunoassay (i-LTC4). In this extraction method, 91% LTC4 was recovered in a final methanol fraction. The identity was confirmed by the recovery test and by the dilution method. The amount of i-LTC4 in plasma from asthmatic patients was determined by radioimmunoassay after the extraction. The order of the plasma level of i-LTC4 was; severe asthma greater than slight or moderate asthma greater than asthmatic patient without attack greater than healthy adult. The highest level of LTC4 was 0.27 +/- 0.11 pmol/ml in severe asthmatic plasma.
肽白三烯（LT），如LTC4、LTD4、LTE4，被认为是速发型超敏反应（如哮喘）的主要介质。我们开发了一种使用Sep-Pak C18柱的快速简便提取方法，用于通过放射免疫测定法（i-LTC4）测定LTC4。在这种提取方法中，91%的LTC4在最终的甲醇馏分中回收。通过回收率测试和稀释法确认了其同一性。哮喘患者血浆中i-LTC4的含量在提取后通过放射免疫测定法测定。i-LTC4的血浆水平顺序为：重度哮喘大于轻度或中度哮喘大于无发作的哮喘患者大于健康成年人。重度哮喘患者血浆中LTC4的最高水平为0.27±0.11 pmol/ml。
