Collins C I, Wesley I V, Murano E A
Iowa State University, Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames, IA 50011.
Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010, USA.
J Food Prot. 1996 May;59(5):448-452. doi: 10.4315/0362-028X-59.5.448.
A modified cefsulodin-irgasan-novobiocin (CIN) medium was developed for the recovery of Arcobacter spp. from meats. Modified CIN was compared to brain heart infusion agar supplemented with 10% bovine blood and cephalothin, vancomycin, and amphotericin B (CVA) as well as brain heart infusion agar supplemented with 10% bovine blood and no antibiotics. The three media were used to recover Arcobacter spp. in a survey of pork-processing plants. Examination of ground pork (149 samples) from one Iowa slaughter facility (Plant #1) revealed that 89 percent of the samples were positive for Arcobacter spp. In a second survey conducted 9 months later involving that same plant and four others, only 5% of the samples from the four plants were found to be positive for Arcobacter spp. Again, 90% of the samples were positive from Plant #1. It was not determined whether the sanitary practices during slaughter or the rearing of pigs on the source farms contributed to the prevalence of Arcobacter spp. in one plant versus another.
开发了一种改良的头孢磺啶-伊红-新生霉素（CIN）培养基，用于从肉类中分离弓形杆菌属细菌。将改良的CIN培养基与补充了10%牛血以及头孢噻吩、万古霉素和两性霉素B的脑心浸液琼脂（CVA），以及补充了10%牛血且无抗生素的脑心浸液琼脂进行比较。在一项对猪肉加工厂的调查中，使用这三种培养基来分离弓形杆菌属细菌。对爱荷华州一家屠宰场（工厂1）的绞碎猪肉（149个样本）进行检测发现，89%的样本弓形杆菌属细菌呈阳性。在9个月后进行的第二项调查中，涉及该工厂及其他四家工厂，结果发现其他四家工厂只有5%的样本弓形杆菌属细菌呈阳性。同样，工厂1的样本中有90%呈阳性。未确定屠宰过程中的卫生措施或源农场猪的饲养方式是否导致了弓形杆菌属细菌在一个工厂与另一个工厂中的不同流行情况。
