Nguyen Paul T, Tuz Karina, Restaino Lawrence, Juárez Oscar
R & F Products, Inc., Downers Grove, IL, United States.
Department of Biology, Illinois Institute of Technology, Chicago, IL, United States.
Front Microbiol. 2022 Jun 21;13:903079. doi: 10.3389/fmicb.2022.903079. eCollection 2022.
species are ubiquitous emerging pathogens with an impact that has been underestimated due to limitations in isolation and detection methods. Our group recently developed the novel NRJ -detection system, with major improvements in specificity and selectivity compared to other culture-based methods. In this work, the NRJ detection system was evaluated using retail whole broiler chicken carcass. Nanopore 16S rRNA gene amplicon sequencing demonstrated that species are found in very low abundance in retail chicken and that indigenous microbiota could be a major factor interfering with detection. Comparison of the microbiome obtained from modified Houf broth (HB) method, as the standard detection system, and the novel NRJ method, showed abundances of <15% and >97%, respectively. The NRJ system significantly inhibits the growth of non-target microbiota, and specifically allows the multiplication of species. In this report, we describe the gold-standard of -specific culture-based method to test food matrices, which can be used for other applications, such as clinical and environmental sampling.
某些物种是普遍存在的新兴病原体，由于分离和检测方法的局限性，其影响一直被低估。我们小组最近开发了新型NRJ检测系统，与其他基于培养的方法相比，在特异性和选择性方面有了重大改进。在这项工作中，使用零售整只肉鸡胴体对NRJ检测系统进行了评估。纳米孔16S rRNA基因扩增子测序表明，在零售鸡肉中某些物种的丰度非常低，并且本地微生物群可能是干扰检测的主要因素。将作为标准检测系统的改良Houf肉汤（HB）方法与新型NRJ方法获得的微生物组进行比较，结果显示某些物种的丰度分别<15%和>97%。NRJ系统显著抑制非目标微生物群的生长，并特别允许某些物种的增殖。在本报告中，我们描述了用于检测食品基质的基于特定培养方法的金标准，该标准可用于其他应用，如临床和环境采样。
