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多形核白细胞迁移对培养的肠上皮单层屏障功能的影响。

Effects of polymorphonuclear leukocyte transmigration on the barrier function of cultured intestinal epithelial monolayers.

作者信息

Nash S, Stafford J, Madara J L

机构信息

Department of Pathology (Gastrointestinal Pathology), Brigham and Women's Hospital, Boston, Massachusetts 02115.

出版信息

J Clin Invest. 1987 Oct;80(4):1104-13. doi: 10.1172/JCI113167.

DOI:10.1172/JCI113167
PMID:3116044
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC442353/
Abstract

We describe a model to study the effects of polymorphonuclear leukocyte (PMN) transmigration on the intestinal epithelial barrier. Human PMN were induced to transmigrate across high resistance monolayers of a cultured human intestinal epithelial cell line (T84 cells) by chemotactic gradients produced by formyl methionyl leucyl phenylalanine (FMLP). With maximal transmigration monolayer resistance decreased by 48 +/- 12.6% in 15 min and by 83 +/- 1.6% in 60 min. This response was dependent on the size of the FMLP gradient and the density of PMN transmigration. The decrease in resistance correlated with number of PMN migrating across monolayers, and was accompanied by increases in flux of paracellular tracers. Macromolecular tracer studies localized the leak sites to foci at which PMN impaled the epithelium. Removal of the chemotactic gradient led to restoration of baseline resistance within 18 h. PMN transmigration across intestinal epithelial monolayers occurs via intercellular occluding junctions and may be associated with a reversible increase in epithelial permeability.

摘要

我们描述了一种用于研究多形核白细胞（PMN）迁移对肠上皮屏障影响的模型。通过甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）产生的趋化梯度，诱导人PMN跨培养的人肠上皮细胞系（T84细胞）的高电阻单层迁移。在最大迁移时，单层电阻在15分钟内降低了48±12.6%，在60分钟内降低了83±1.6%。这种反应取决于FMLP梯度的大小和PMN迁移的密度。电阻的降低与跨单层迁移的PMN数量相关，并伴有细胞旁示踪剂通量的增加。大分子示踪剂研究将渗漏部位定位在PMN刺入上皮的部位。去除趋化梯度导致在18小时内恢复基线电阻。PMN跨肠上皮单层的迁移通过细胞间紧密连接发生，并且可能与上皮通透性的可逆增加有关。

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