Dharmsathaphorn K, McRoberts J A, Mandel K G, Tisdale L D, Masui H
Am J Physiol. 1984 Feb;246(2 Pt 1):G204-8. doi: 10.1152/ajpgi.1984.246.2.G204.
A human colonic epithelial cell line, T84, derived from a colonic carcinoma, has been examined both morphologically and functionally. The cells grew to confluence as a monolayer with the basolateral membrane attached to the surface of the culture dish and a microvillus-studded apical membrane facing the media. Tight junctions and desmosomes were demonstrated between adjacent cells. Confluent monolayer cultures conducted vectorial electrolyte transport that could be altered by a variety of secretagogues and antisecretagogues similar to isolated intestinal tissues. This cell line will serve as an excellent model system for the study of electrolyte transport processes and their regulation by peptide hormones and neurotransmitters.
一种源自结肠癌的人结肠上皮细胞系T84，已在形态学和功能方面进行了检测。这些细胞以单层形式生长至汇合状态，其基底外侧膜附着于培养皿表面，微绒毛密布的顶端膜面向培养基。相邻细胞之间可见紧密连接和桥粒。汇合的单层培养物进行向量电解质转运，这种转运可被多种促分泌剂和抗分泌剂改变，类似于分离的肠组织。该细胞系将作为研究电解质转运过程及其受肽类激素和神经递质调节的极佳模型系统。
