Lanham Stuart A, DuPriest Elizabeth, Kupfer Philipp, Cooper Cyrus, Bagby Susan P, Oreffo Richard O C
Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK.
Division of Nephrology & Hypertension, Oregon Health & Science University and Portland VA Medical Center, Portland, Oregon.
Physiol Rep. 2019 Jun;7(11):e14081. doi: 10.14814/phy2.14081.
Epidemiological studies suggest skeletal growth is programmed during intrauterine and early postnatal life. We hypothesize that bone development may be altered by maternal diet and have investigated this using a microswine model of maternal protein restriction (MPR). Mothers were fed a control diet (14% protein) or isocaloric low (1%) protein diet during late pregnancy and for 2 weeks postnatally. Offspring were weaned at 4 weeks of age to ad lib or calorie-restricted food intake groups. Femur and vertebra were analysed by micro computed tomography in offspring 3-5 months of age. Caloric restriction from 4 weeks of age, designed to prevent catch-up growth, showed no significant effects on bone structure in the offspring from either maternal dietary group. A maternal low protein diet altered trabecular number in the proximal femur and vertebra in juvenile offspring. Cortical bone was unaffected. These results further support the need to understand the key role of the nutritional environment in early development on programming of skeletal development and consequences in later life.
流行病学研究表明，骨骼生长在子宫内和出生后早期就已编程。我们假设母亲的饮食可能会改变骨骼发育，并使用母体蛋白质限制（MPR）的微型猪模型对此进行了研究。在妊娠晚期和产后2周，给母猪喂食对照饮食（14%蛋白质）或等热量低（1%）蛋白质饮食。后代在4周龄时断奶，分为自由采食或热量限制食物摄入组。对3至5月龄后代的股骨和椎骨进行了微型计算机断层扫描分析。从4周龄开始的热量限制旨在防止追赶生长，对来自任何一个母体饮食组的后代的骨骼结构均无显著影响。母体低蛋白饮食改变了幼年后代近端股骨和椎骨的小梁数量。皮质骨未受影响。这些结果进一步支持了有必要了解早期发育中营养环境对骨骼发育编程及后期生活后果的关键作用。
