Nakamura K, Chiao J W, Nagamatsu G R, Addonizio J C
Dept. of Urology, New York Medical College, Valhalla 10595.
J Urol. 1987 Oct;138(4):867-70. doi: 10.1016/s0022-5347(17)43742-6.
The role of monocytes in cell-mediated cytolysis of bladder cancer cells was investigated. Human peripheral monocytes released a cytolytic factor which lysed T24 bladder cancer cells and a number of human tumor cells, but not normal lymphocytes or fibroblasts. After incubation of monocytes with bacillus Calmette-Guerin for 48 hr. in vitro, cytolysis of T24 cells was increased up to 56.7 +/- 4.1%. Treatment of monocytes with actinomycin D (an inhibitor of RNA transcription) reduced release of cytolytic factor from 27.3 +/- 5.7% cytolysis to 4.5 +/- 1.4% (p less than 0.05). The response to mitomycin C was different between lymphokines and monocyte cytolytic factor. The mouse monoclonal antibody against human recombinant tumor necrosis factor did not neutralize monocyte cytolytic factor. These results show that this monocyte cytolytic factor is distinct from lymphokines and tumor necrosis factor. The evidence that bacillus Calmette-Guerin increases release of monocyte cytolytic factor may be associated with anti-tumor activity of bacillus Calmette-Guerin in intravesical therapy for treatment of bladder cancer.
研究了单核细胞在膀胱癌细胞介导的细胞溶解中的作用。人外周血单核细胞释放一种细胞溶解因子，该因子可溶解T24膀胱癌细胞和多种人类肿瘤细胞，但对正常淋巴细胞或成纤维细胞无作用。单核细胞与卡介苗在体外孵育48小时后，T24细胞的细胞溶解率增加至56.7±4.1%。用放线菌素D（一种RNA转录抑制剂）处理单核细胞，可使细胞溶解因子的释放从27.3±5.7%的细胞溶解率降至4.5±1.4%（p<0.05）。淋巴因子和单核细胞细胞溶解因子对丝裂霉素C的反应不同。抗人重组肿瘤坏死因子的小鼠单克隆抗体不能中和单核细胞细胞溶解因子。这些结果表明，这种单核细胞细胞溶解因子与淋巴因子和肿瘤坏死因子不同。卡介苗增加单核细胞细胞溶解因子释放的证据可能与卡介苗在膀胱癌膀胱内治疗中的抗肿瘤活性有关。
