Department of Biophysics, Panjab University Chandigarh, India.
Department of Biophysics, Panjab University Chandigarh, India.
Food Chem Toxicol. 2019 Sep;131:110551. doi: 10.1016/j.fct.2019.05.059. Epub 2019 Jun 1.
Tea is a natural resource of catechins and exhibits antioxidative and anticancer activities. This study was designed to elucidate the comparative efficacy of white tea and pure EGCG in containing benzo (a) pyrene (BaP)-induced pulmonary stress. Rats were treated with white tea extract (WT) (1%) and pure EGCG at a dose of 80μg/ml in drinking water on alternate days for 12 weeks (4 weeks prior, during and after BaP treatment). BaP(50 mg/kg b. wt) was administered to rats orally in olive oil twice a week for four weeks. The indices such as stress biomarkers (LPO, PCC & ROS), antioxidant enzymes (SOD, CAT, GSH, GST, GR, GPx) activities and lung histoarchitecture were assessed. BaP administration enhanced the levels of inflammatory markers (NO and citrulline) and reduced activities of antioxidant enzymes. We observed similar antioxidant efficacy by both WT and EGCG as seen by their ameliorative action in restoring BaP induced oxidative and inflammatory stress as well as lung histoarchitecture. Our findings suggest that WT is equally beneficial as EGCG in maintaining the integrity of alveoli and is a potential candidate to be used as a cost effective and protective agent in conditions of BaP-induced lung damage.
茶是儿茶素的天然资源,具有抗氧化和抗癌活性。本研究旨在阐明白茶和纯 EGCG 在缓解苯并(a)芘(BaP)诱导的肺部应激方面的比较效果。大鼠在饮用水中分别用白茶提取物(WT)(1%)和纯 EGCG(80μg/ml)进行处理,每隔一天处理一次,共 12 周(BaP 处理前、处理期间和处理后各 4 周)。BaP(50mg/kg b.wt)以橄榄油形式每周两次口服给予大鼠,共 4 周。评估应激生物标志物(LPO、PCC 和 ROS)、抗氧化酶(SOD、CAT、GSH、GST、GR 和 GPx)活性和肺组织形态等指标。BaP 处理增强了炎症标志物(NO 和瓜氨酸)的水平,并降低了抗氧化酶的活性。我们观察到 WT 和 EGCG 具有相似的抗氧化功效,它们通过恢复 BaP 诱导的氧化和炎症应激以及肺组织形态来发挥其改善作用。我们的研究结果表明,WT 与 EGCG 一样有助于维持肺泡的完整性,是一种有潜力的候选物,可作为 BaP 诱导的肺部损伤情况下的经济有效和保护剂。