Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraidah 52571, Saudi Arabia.
Department of Basic Health Sciences, College of Applied Medical Science, Qassim University, Buraidah 52571, Saudi Arabia.
Molecules. 2020 Feb 7;25(3):724. doi: 10.3390/molecules25030724.
Benzo(a)pyrene (BaP) is a well-known carcinogen and enhances oxidative stress and apoptosis and also alters several molecular pathways. Curcumin is an active ingredient of , and it has potent anti-inflammatory, antioxidant activity that defends cells from oxidative stress and cell death. The objectives of the present study were to explore the protective effects of curcumin against long-term administration of BaP induced disturbances in lungs of rats. Male rats were randomly divided into four groups: saline control, BaP only, BaP + curcumin, and curcumin only. Lung histopathology, electron microscopy, inflammatory cytokine release, antioxidant levels, apoptosis, and cell cycle were examined. Instillation of BaP significantly increased infiltration of inflammatory cells in alveolar space and inflammatory cytokine in blood. BaP induced lung tissue alterations including mild bronchitis, scant chronic inflammatory cell infiltrate in the wall of the respiratory bronchiole, and mild intra-alveolar haemorrhage. However, these alterations were found to be significantly less as mild inflammatory cell infiltrate in curcumin plus BaP treated group. Furthermore, electron microscopy results also showed necrotic changes and broken cell membrane of Type-II epithelial cell of alveoli in BaP group, which was reduced after adding curcumin treatment. In addition, we found BaP plus curcumin treatment effectively reduced inflammatory cytokines Tumour Necrosis Factor alpha (TNF-α), Interleukin 6 (IL-6), and C-reactive protein (CRP) levels in blood serum. Moreover, the levels of tunnel staining and p53 expression were significantly increased by BaP, whereas these changes were noticeably modulated after curcumin treatment. BaP also interferes in normal cell cycle, which was significantly improved with curcumin treatment. Overall, our findings suggest that curcumin attenuates BaP -induced lung injury, probably through inhibiting inflammation, oxidative stress and apoptosis in lung epithelial cells, and improving cell proliferation and antioxidants level. Thus, curcumin may be an alternative therapy for improving the outcomes of Benzo(a)pyrene-induced lung injury.
苯并(a)芘（BaP）是一种众所周知的致癌物质，它会增强氧化应激和细胞凋亡，并改变几种分子途径。姜黄素是 的一种活性成分，具有强大的抗炎、抗氧化活性，可以保护细胞免受氧化应激和细胞死亡。本研究的目的是探讨姜黄素对长期给予 BaP 诱导的大鼠肺部紊乱的保护作用。雄性大鼠随机分为四组：生理盐水对照组、BaP 组、BaP+姜黄素组和姜黄素组。检查了肺组织病理学、电子显微镜、炎性细胞因子释放、抗氧化水平、细胞凋亡和细胞周期。BaP 的注入显著增加了肺泡空间中的炎症细胞浸润和血液中的炎症细胞因子。BaP 诱导的肺组织改变包括轻度支气管炎、呼吸细支气管壁中慢性炎症细胞浸润减少和轻度肺泡内出血。然而，在姜黄素加 BaP 处理组中发现这些改变明显较轻，为轻度炎症细胞浸润。此外，电子显微镜结果还显示 BaP 组肺泡型 II 上皮细胞出现坏死变化和细胞膜破裂，而添加姜黄素治疗后这种情况减少。此外，我们发现 BaP+姜黄素治疗可有效降低血清中肿瘤坏死因子-α（TNF-α）、白细胞介素 6（IL-6）和 C 反应蛋白（CRP）等炎症细胞因子的水平。此外，BaP 可显著增加隧道染色和 p53 表达，而姜黄素治疗后这些变化明显得到调节。BaP 还干扰正常细胞周期，姜黄素治疗可显著改善。总的来说，我们的研究结果表明，姜黄素可减轻 BaP 诱导的肺损伤，可能是通过抑制肺上皮细胞的炎症、氧化应激和细胞凋亡，以及改善细胞增殖和抗氧化剂水平。因此，姜黄素可能是改善苯并(a)芘诱导的肺损伤的一种替代疗法。
BMC Pharmacol Toxicol. 2020-8-18
Toxicol Mech Methods. 2011-8-23
Biomedicines. 2024-6-18
Pharmaceuticals (Basel). 2023-3-16
Biology (Basel). 2022-2-26
Int J Mol Sci. 2022-1-28
J Mol Med (Berl). 2020-1
Mediators Inflamm. 2019-8-21
Zotero 插件
