Department of Preventive Dentistry, Periodontology and Cariology, University Medical Center Göttingen, Göttingen, Germany,
Department of Preventive Dentistry, Periodontology and Cariology, University Medical Center Göttingen, Göttingen, Germany.
Caries Res. 2019;53(6):636-642. doi: 10.1159/000500046. Epub 2019 Jun 4.
This study aimed to analyse if the erosion-protective potential of the salivary pellicle is different between female and male subjects. Bovine enamel and dentin specimens (each n = 3) were exposed to the oral cavity of healthy female or male volunteers (each n = 25, females: 25.8 ± 3.5 years, males: 26.7 ± 4.0 years) for 120 min to form a salivary pellicle. Subsequently, each 2 enamel and 2 dentin specimens were eroded with hydrochloric acid (pH 2.6, 60 s). Specimens of the control group (each n = 30) were eroded without presenting a salivary pellicle. Calcium release into the acid was determined photometrically. Additionally, total protein content in the pellicle (each n = 1 enamel and dentin specimen/volunteer) and different salivary parameters (flow rate, pH, buffer capacity, protein, albumin, calcium, phosphate, fluoride) were assessed. Statistical analyses were performed by one-way ANOVA, t tests, multiple linear regressions and Pearson correlations (p < 0.05). The erosion-protective capacity was not significantly different among female (calcium release [% of control]: enamel: 82.6 ± 28.1, dentin: 80.7 ± 24.0) and male (enamel: 76.0 ± 27.5, dentin: 87.1 ± 34.9) subjects. The protein content of the pellicle was not different between female and male subjects. The protein content and pH of unstimulated saliva were significantly reduced in female compared to male volunteers. Calcium release was neither correlated with the protein content of the salivary pellicle nor with salivary parameters. Under the conditions of the present study, the erosion-protective capacity of the salivary pellicle of female and male subjects is not different.
本研究旨在分析唾液膜的抗蚀潜力在女性和男性受试者之间是否存在差异。将牛牙釉质和牙本质标本（各 n = 3）暴露于健康女性或男性志愿者（各 n = 25，女性：25.8 ± 3.5 岁，男性：26.7 ± 4.0 岁）的口腔中 120 分钟以形成唾液膜。随后，用盐酸（pH 2.6，60 秒）对每个 2 个牙釉质和 2 个牙本质标本进行腐蚀。对照组（各 n = 30）的标本在不呈现唾液膜的情况下进行腐蚀。通过光度法测定酸中钙的释放量。此外，评估了膜（各 n = 1 个牙釉质和牙本质标本/志愿者）和不同唾液参数（流速、pH 值、缓冲能力、蛋白质、白蛋白、钙、磷酸盐、氟化物）中的总蛋白含量。通过单向方差分析、t 检验、多元线性回归和 Pearson 相关性（p < 0.05）进行统计分析。女性（钙释放 [%对照]：牙釉质：82.6 ± 28.1，牙本质：80.7 ± 24.0）和男性（牙釉质：76.0 ± 27.5，牙本质：87.1 ± 34.9）受试者之间的抗蚀能力没有显著差异。膜中的蛋白质含量在女性和男性受试者之间没有差异。与男性志愿者相比，女性未刺激唾液的蛋白质含量和 pH 值显著降低。钙释放与唾液膜的蛋白质含量或唾液参数均无相关性。在本研究的条件下，女性和男性受试者的唾液膜的抗蚀能力没有差异。
