牙体侵蚀性丧失取决于釉质表面结构和薄膜的存在——一项体外研究。

Erosive loss of tooth substance is dependent on enamel surface structure and presence of pellicle - An in vitro study.

机构信息

Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, Switzerland.

Clinic of Cranio-Maxillofacial and Oral Surgery, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, Switzerland.

出版信息

Arch Oral Biol. 2020 Apr;112:104686. doi: 10.1016/j.archoralbio.2020.104686. Epub 2020 Feb 21.

DOI:10.1016/j.archoralbio.2020.104686

PMID:32112967

Abstract

OBJECTIVE

Aim of this in vitro study was to investigate erosive tooth loss in dependence of the enamel surface structure and presence of an acquired pellicle.

METHODS

Enamel specimens from 19 bovine incisors (4 specimens/incisor) were allocated to four experimental groups (n = 19). The surfaces of half of the specimens were polished (two groups), while the other half was left native (two groups). Specimens of one polished and one native group were placed in pooled human saliva (30 min) for the formation of an acquired pellicle. Thereafter, all specimens were demineralized by superfusion with hydrochloric acid (17 min, pH 2.3) with collection of the superfluent. Erosive substance loss was determined by measuring the dissolved calcium content using a colorimetric assay with Arsenazo III reagent. Differences in erosive substance loss were statistically analyzed with respect to enamel surface and pellicle. A linear mixed effects model was fitted to the data and pairwise differences between groups were evaluated (significance level α= 0.05).

RESULTS

Enamel surface structure (p < 0.001) and presence of pellicle (p = 0.01) had a significant effect on erosive substance loss. Polished surfaces with pellicle showed the lowest cumulative calcium release [nmol Ca/mm] (means ± standard deviation: 48+/-5), followed by polished specimens without (51+/-9) and native specimens with pellicle (54+/-10). No significant differences were found between these groups. Highest cumulative calcium release was found for native specimens without pellicle (61+/-9; p < 0.05).

CONCLUSIONS

Both enamel surface structure and the acquired pellicle are important determinants of the susceptibility to erosive tooth loss.

摘要

目的

本体外研究旨在探讨牙釉质表面结构和获得性黏膜对侵蚀性牙丧失的影响。

方法

从 19 颗牛切牙中取出 19 个牙釉质标本（每个切牙 4 个标本），将其分为 4 个实验组（n = 19）。一半标本的表面进行抛光（两组），另一半保留自然状态（两组）。抛光组和自然组的一半标本被放入混合的人唾液中（30 分钟）以形成获得性黏膜。然后，所有标本均用盐酸（17 分钟，pH 值 2.3）超滤液进行脱矿处理，并收集超滤液。采用 Arsenazo III 试剂比色法测定溶解钙含量，以确定侵蚀性物质的损失。用线性混合效应模型对数据进行分析，并评估组间差异（显著性水平α= 0.05）。

结果

牙釉质表面结构（p < 0.001）和黏膜的存在（p = 0.01）对侵蚀性物质的损失有显著影响。有黏膜的抛光表面显示出最低的累积钙释放量[nmol Ca/mm]（平均值 ± 标准差：48±5），其次是无黏膜的抛光标本（51±9）和有黏膜的自然标本（54±10）。这些组之间没有显著差异。无黏膜的自然标本的累积钙释放量最高（61±9；p < 0.05）。