Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz 5138665793, Iran.
Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz 5157944533, Iran.
Biomolecules. 2019 Jun 3;9(6):215. doi: 10.3390/biom9060215.
This paper aimed to evaluate the effect of herbal mixture (, , , and ) only and along with clomiphene citrate (CC) compared to CC on serum antioxidants, glycemic status, menstrual regulation, and rate of pregnancy. This single-blind randomized clinical trial was carried out on 60 infertile participants with polycystic ovary syndrome (PCOS) willing to be pregnant. They were randomly allocated into group 1 ( = 20) who received routine dose of CC pills (50-150 mg) for three menstrual cycles from the fifth day of menstruation for five days; group 2 ( = 20) who consumed herbal mixture daily (700 mg); and group 3 ( = 20) who used up herbal mixture along with CC for 3 months. Catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA), fasting blood sugar (FBS), insulin, and homeostatic model assessment for insulin resistance (HOMA-IR) were measured in their blood samples. No statistically significant differences were observed between the three groups in terms of socio-demographic characteristics. After intervention, however, the levels of CAT in group 2 (adjusted mean difference (aMD): = 9.0; confidence interval (CI) 95% = 1.1-16.9) and group 3 (aMD = 12.2; CI 95% = 4.5-19.9), GPx in group 2 (aMD = 986.1; CI 95% = 141.1-1831.1) and group 3 (aMD = 1781.2; CI 95% = 960.7-2601.8), and SOD in group 2 (aMD = 55.1; CI 95% = 26.0-84.2) increased. While FBS in group 3 (aMD = -8.7; CI 95% = -14.7 to -2.7), insulin in group 2 (aMD = -5.6; CI 95% = -10.8 to -0.4), and HOMA-IR in group 2 (aMD = -1.3; CI 95% = -2.4 to -0.2) significantly decreased compared to the group 1. To summarize, herbal mixture supplements along with CC have beneficial effects on serum antioxidant levels, as well as glycemic biomarkers of infertile PCOS, menstrual regulation, and pregnancy rate.
本研究旨在评估草药混合物（、、、和）单独使用以及与枸橼酸氯米酚（CC）联合使用对血清抗氧化剂、血糖状态、月经调节和妊娠率的影响。这是一项单盲随机临床试验，共纳入 60 名有生育意愿的多囊卵巢综合征（PCOS）不孕患者。他们被随机分为三组：第 1 组（n=20）接受常规剂量的 CC 片剂（50-150mg），从月经第 5 天开始连续服用 3 个周期，每天 5 天；第 2 组（n=20）每天服用草药混合物；第 3 组（n=20）同时服用草药混合物和 CC，共 3 个月。检测他们的血液样本中的过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）、丙二醛（MDA）、空腹血糖（FBS）、胰岛素和稳态模型评估的胰岛素抵抗（HOMA-IR）。然而，在干预后，第 2 组的 CAT 水平（调整平均差异（aMD）：=9.0；95%置信区间（CI）=1.1-16.9）和第 3 组（aMD=12.2；95%CI=4.5-19.9）、第 2 组的 GPx 水平（aMD=986.1；95%CI=141.1-1831.1）和第 3 组（aMD=1781.2；95%CI=960.7-2601.8）以及第 2 组的 SOD 水平（aMD=55.1；95%CI=26.0-84.2）均升高。而第 3 组的 FBS（aMD=-8.7；95%CI=-14.7 至-2.7）、第 2 组的胰岛素（aMD=-5.6；95%CI=-10.8 至-0.4）和第 2 组的 HOMA-IR（aMD=-1.3；95%CI=-2.4 至-0.2）与第 1 组相比均显著降低。总之，草药混合物补充剂与 CC 联合使用对血清抗氧化剂水平以及不孕 PCOS 的血糖生物标志物、月经调节和妊娠率有有益影响。
