Brierley Sam E, Adlam Anthony R, Hall Matthew K D
The New Zealand Institute for Plant & Food Research Limited, Private Bag 11600, Palmerston North 4442, New Zealand.
Apreso, PO Box 2407, Palmerston North 4442, New Zealand.
Methods Protoc. 2018 Dec 20;2(1):1. doi: 10.3390/mps2010001.
Compared to previously tested fumigants such as methyl bromide, sulfuryl fluoride and phosphine; ethanedinitrile (EDN) is a new fumigant which is being trialled around the world as a pre-plant soil treatment and as a quarantine and pre-shipment (QPS) treatment of commodities. To collect the data necessary to assess the effectiveness of this fumigant, an accurate analytical method is needed across a wide concentration range. We reviewed the methods of detection for EDN described in recently published fumigation studies and have developed and validated a method to quantify EDN in air using a gas chromatograph equipped with a flame ionization detector (GC-FID). Our tested method has a linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) of R 0.9988, 1.36%, 98.8%, 0.750 ppm and 1.073 ppm, respectively. These values were determined using internationally recognised guidelines for the validation of non-standard analytical methods, which means that our method can be applied to the different validation requirements of regulatory agencies and countries. Our method can be used for experimental conditions that require detection at low and high concentrations simultaneously because it is accurate, fast (0.6 min) and repeatable across a concentration range of 1 to 40,000 ppm. This method will help to standardise the quantification of EDN by research groups and facilitate acceptance of data by regulatory organisations around the world.
与之前测试过的熏蒸剂如溴甲烷、硫酰氟和磷化氢相比，乙二腈（EDN）是一种新型熏蒸剂，正在全球范围内作为种植前土壤处理以及商品检疫和装运前（QPS）处理进行试验。为了收集评估这种熏蒸剂有效性所需的数据，需要一种适用于广泛浓度范围的准确分析方法。我们回顾了近期发表的熏蒸研究中描述的EDN检测方法，并开发并验证了一种使用配备火焰离子化检测器（GC-FID）的气相色谱仪对空气中的EDN进行定量的方法。我们测试的方法的线性、精密度、准确度、检测限（LOD）和定量限（LOQ）分别为R 0.9988、1.36%、98.8%、0.750 ppm和1.073 ppm。这些值是根据国际认可的非标准分析方法验证指南确定的，这意味着我们的方法可以应用于监管机构和不同国家的不同验证要求。我们的方法可用于需要同时检测低浓度和高浓度的实验条件，因为它准确、快速（0.6分钟）且在1至40,000 ppm的浓度范围内可重复。该方法将有助于研究小组对EDN定量进行标准化，并促进全球监管组织对数据的认可。
