Ryu Meguya, Honda Reo, Cernescu Adrian, Vailionis Arturas, Balčytis Armandas, Vongsvivut Jitraporn, Li Jing-Liang, Linklater Denver P, Ivanova Elena P, Mizeikis Vygantas, Tobin Mark J, Morikawa Junko, Juodkazis Saulius
Tokyo Institute of Technology, Meguro-ku, Tokyo 152-8550, Japan.
Neaspec GmbH, Bunsenstrasse 5, 82152 Martinsried, Germany.
Beilstein J Nanotechnol. 2019 Apr 23;10:922-929. doi: 10.3762/bjnano.10.93. eCollection 2019.
The nanoscale composition of silk defining its unique properties via a hierarchial structural anisotropy needs to be analysed at the highest spatial resolution of tens of nanometers corresponding to the size of fibrils made of β-sheets, which are the crystalline building blocks of silk. Nanoscale optical and structural properties of silk have been measured from 100 nm thick longitudinal slices of silk fibers with ca. 10 nm resolution, the highest so far. Optical sub-wavelength resolution in hyperspectral mapping of absorbance and molecular orientation were carried out for comparison at IR wavelengths of 2-10 μm using synchrotron radiation. A reliable distinction of transmission changes by only 1-2% as the anisotropy of amide bands was obtained from nanometer-thin slices of silk.
丝绸的纳米级组成通过层次结构各向异性决定其独特性能，需要在与由β-折叠片构成的原纤维尺寸相对应的数十纳米的最高空间分辨率下进行分析，β-折叠片是丝绸的晶体结构单元。已从约100纳米厚的丝绸纤维纵向切片中测量了丝绸的纳米级光学和结构特性，分辨率约为10纳米，这是迄今为止的最高分辨率。利用同步辐射在2-10μm的红外波长下进行了吸光度和分子取向的高光谱映射中的光学亚波长分辨率测量，以作比较。从纳米薄的丝绸切片中获得了酰胺带各向异性仅1-2%的透射变化的可靠区分。
