验证双着丝粒染色体分析在韩国的辐射生物剂量学中的应用。
Validation of the dicentric chromosome assay for radiation biological dosimetry in South Korea.
机构信息
Laboratory of Biological Dosimetry, National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
National Radiation Emergency Medical Center, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea.
出版信息
J Radiat Res. 2019 Oct 23;60(5):555-563. doi: 10.1093/jrr/rrz039.
The dicentric chromosome assay (DCA) is a well-established biodosimetry test to estimate exposure to ionizing radiation. The Korea Institute of Radiological and Medical Sciences (KIRAMS) established a DCA protocol as a medical response to radiation emergencies in South Korea. To maintain its accuracy and performance, intercomparison exercises with Health Canada (HC) have been conducted; herein, we aimed to validate our capacity of DCA analysis based on those results. Blood samples irradiated at HC were shipped to KIRAMS to assess the irradiation dose to blinded samples using conventional DCA full scoring and triage-based techniques (conventional DCA scoring in triage mode and DCA QuickScan method). Actual doses fell within the 95% confidence intervals of dose estimates for 70-100% of the blinded samples in 2015-2018. All methods discriminated binary dose categories, reflecting clinical significance. This DCA can be used as a reliable radiation biodosimetry tool in preparation for radiation accidents in South Korea.
着丝粒染色体分析（DCA）是一种成熟的生物剂量测定方法，可用于估计电离辐射暴露情况。韩国放射医学研究所（KIRAMS）制定了 DCA 方案，作为韩国辐射紧急情况的医疗应对措施。为了保持其准确性和性能，与加拿大卫生部（HC）进行了国际比较活动；在此，我们旨在根据这些结果验证我们的 DCA 分析能力。在 HC 处辐照的血液样本被运送到 KIRAMS，使用传统的 DCA 全评分和基于分类的技术（分类 DCA 评分模式和 DCA QuickScan 方法）对盲样的照射剂量进行评估。在 2015-2018 年，实际剂量落在 70-100%盲样剂量估计值的 95%置信区间内。所有方法都能区分二进制剂量类别，反映出临床意义。这种 DCA 可作为韩国辐射事故准备的可靠辐射生物剂量学工具。
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