中国22家实验室之间的细胞遗传学剂量测定比对

Intercomparison in Cytogenetic Dosimetry among 22 Laboratories in China.

作者信息

Liu Jian Xiang, Pan Yan, Ruan Jian Lei, Piao Chunnan, Su Xu

机构信息

Chinese Center for Disease Control and Prevention Key Laboratory of Radiological Protection and Nuclear Emergency, Beijing 100088, China; National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China; Chinese Center for Medical Response to Radiation Emergency, Ministry of Health, Beijing 100088, China.

出版信息

Genome Integr. 2016 Dec 30;7:6. doi: 10.4103/2041-9414.197164. eCollection 2016.

DOI:10.4103/2041-9414.197164

PMID:28217282

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5292918/

Abstract

As part of a regional International Atomic Energy Agency-coordinated research project with the support from the National Health and Family Planning Commission of China, 22 laboratories participated in the intercomparison in cytogenetic dosimetry in China. Slides for chromosomal aberrations were prepared by the Department of Radiation Epidemiology, National Institute for Radiological Protection, which organized the exercise. Slides were sent to the other participating laboratories through Express Mail Service. For estimates of dose, each laboratory scored the frequency of dicentrics plus centric rings chromosomes. The whole blood samples were irradiated with Co γ-rays (1.3 Gy, 2.4 Gy and 1.5 Gy, 2.6 Gy). Each laboratory got one group of the slides. Ten of the 44 estimates of dose fell within ±5% of the true physical dose, 12 fell within ±5-10%, 9 fell within ±10-15%, 12 fell within ±15-20%, while only one sample fell ± >20%. The evaluation of the respective dose was achieved by 21 laboratories.

摘要

作为由国际原子能机构协调、在中国国家卫生和计划生育委员会支持下开展的一个地区性研究项目的一部分，22个实验室参与了中国细胞遗传学剂量测定的比对。用于染色体畸变分析的玻片由组织此次活动的中国国家放射防护研究所辐射流行病学部制备。玻片通过特快专递寄送给其他参与实验室。为了估算剂量，每个实验室对双着丝粒染色体和着丝粒环染色体的频率进行评分。全血样本用钴γ射线（1.3 Gy、2.4 Gy和1.5 Gy、2.6 Gy）进行照射。每个实验室收到一组玻片。44个剂量估算值中有10个落在真实物理剂量的±5%范围内，12个落在±5-10%范围内，9个落在±10-15%范围内，12个落在±15-20%范围内，而只有一个样本的误差超过±20%。21个实验室完成了各自剂量的评估。