Sun Ye, Wang Hao, Zhang Tao, Liu Wei-Hong, Liu Hong-Yu, Yu Li-Yan, Zhang Yu-Qin
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali 671003, PR China.
Int J Syst Evol Microbiol. 2019 Aug;69(8):2486-2491. doi: 10.1099/ijsem.0.003519. Epub 2019 Jun 6.
The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711, was established using a polyphasic approach. Cells of the isolate were Gram-staining-positive, aerobic, non-motile cocci. Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v). Galactose, arabinose and ribose were detected as the sugar compositions in the whole cell hydrolysates. The peptidoglycan type was A3gamma (ll-Dpm-Gly). MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids. The predominant fatty acid was anteiso-C15 : 0. The genomic DNA G+C content was 73.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711 affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617. Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711 represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp. nov. is proposed, with CPCC 204711 (=KCTC 39977=DSM 105431) as the type strain.
采用多相分类方法确定了从巴丹吉林沙漠采集的沙漠土壤样品中分离出的一株放线菌（命名为CPCC 204711）的分类地位。该分离株的细胞革兰氏染色阳性，好氧，无运动性球菌。在28°C（范围为20 - 40°C）、pH 7.0（范围为pH 6.0 - 8.0）和0 - 1%（范围为0 - 5%，w/v）NaCl浓度下观察到良好生长。在全细胞水解物中检测到半乳糖、阿拉伯糖和核糖作为糖成分。肽聚糖类型为A3γ（ll-Dpm-Gly）。检测到MK-9(H4)作为主要的甲基萘醌，双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、几种未鉴定的糖脂和一种未鉴定的氨基糖脂作为主要的极性脂质。主要脂肪酸为anteiso-C15 : 0。基因组DNA G+C含量为73.1 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株CPCC 204711隶属于丙酸杆菌科，该菌株在Mariniluteicoccus属旁边形成了一个独特的系统发育谱系，与内生Mariniluteicoccus endophyticus YIM 2617的16S rRNA基因序列相似性最高为96.0%。系统发育分析和表型特征均支持菌株CPCC 204711代表丙酸杆菌科中一个新属的新物种，为此提出新属名为Desertihabitans，新种名为Desertihabitans aurantiacus gen. nov., sp. nov.，以CPCC 204711（=KCTC 39977=DSM 105431）作为模式菌株。
