Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Int J Syst Evol Microbiol. 2020 Feb;70(2):1166-1171. doi: 10.1099/ijsem.0.003896.
A novel actinobacterium, designated strain 16Sb5-5, was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China. The strain was examined by a polyphasic approach to clarify its taxonomic position. Cells of the isolate were Gram-staining-positive, aerobic, non-motile and short-rod shaped. Strain 16Sb5-5 grew optimally at 37 °C, pH 7.0 and with 0‒2 % (w/v) NaCl. The cell-wall peptidoglycan was of the A3 type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP). Ribose, arabinose and glucose were detected in the whole-cell hydrolysates. The predominant menaquinone was MK-9(H). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid, three unidentified glycolipids and three unidentified lipids. The major whole-cell fatty acids were anteiso-C and iso-C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5 was closely related to CPCC 204711 (99.8 % similarity) and formed a robust clade with in the phylogenetic trees. genomic comparisons showed that strain 16Sb5-5 exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to CPCC 204711. The genomic G+C content was 73.3 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5 could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 16Sb5-5 (=KCTC 49116=CGMCC 1.16553). The description of the genus has also been emended.
一株新的放线菌，命名为 16Sb5-5 菌株，从中国新疆维吾尔自治区塔克拉玛干沙漠的沙样中分离得到。该菌株通过多相分析进行了研究，以阐明其分类地位。分离株的细胞革兰氏染色阳性，需氧，不运动，呈短杆状。16Sb5-5 菌株最佳生长温度为 37℃，pH 值为 7.0，NaCl 浓度为 0-2%（w/v）。细胞壁肽聚糖属 A3 型，含有丙氨酸、甘氨酸、谷氨酸和 ll-二氨基庚二酸（ll-DAP）。全细胞水解物中检测到核糖、阿拉伯糖和葡萄糖。主要的menaquinone 是 MK-9(H)。极性脂谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、一种未鉴定的磷脂、三种未鉴定的糖脂和三种未鉴定的脂类组成。全细胞脂肪酸主要为 anteiso-C 和 iso-C。基于 16S rRNA 基因序列的系统发育分析表明，16Sb5-5 菌株与 CPCC 204711（相似度 99.8%）密切相关，并与 在系统发育树中形成一个坚固的分支。基因组比较表明，16Sb5-5 菌株与 CPCC 204711 的 ANI 值为 94.8-94.9%，GGDC 值为 59.5%。基因组 G+C 含量为 73.3mol%。基于系统发育、表型和化学分类分析，16Sb5-5 菌株与最接近的系统发育相关物不同，代表了属的一个新种，建议命名为 sp. nov.。模式菌株为 16Sb5-5（=KCTC 49116=CGMCC 1.16553）。对 属的描述也进行了修订。
