Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, PR China.
Department of Microbiology and Molecular Genetics, University of the Punjab, Quid-i-Azam Campus, Lahore 54590, Pakistan.
Int J Syst Evol Microbiol. 2020 May;70(5):3179-3185. doi: 10.1099/ijsem.0.004152.
A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435, was isolated from soil sample collected from the Cholistan Desert, Pakistan. The taxonomic position of the strain was established by using a polyphasic taxonomic approach. The cells were coccoid-shaped and found in single or arrangement of pairs. The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %). Results of blast analysis based on 16S rRNA gene sequences showed that MON 2.2 was its closest relative with 97.4 % similarity followed by CPCC 204711 (95.2 %). In phylogenetic trees, strain F435 formed a robust cluster with the only member of the genus . The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid. The major fatty acid was determined to be anteiso-C. Characteristic polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids and glycolipids. The predominant menaquinone was MK-9(H). The genomic G+C content was calculated as 73.5 mol%. The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435 and MON 2.2 were 24.6 and 81.8 %, respectively. Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435 represents a novel specie of the genus , for which the name sp. nov. is proposed. The type strain is F435 (=JCM 33648=CGMCC 1.17443). The description of the genus has also been emended.
一株革兰氏阳性、需氧、非运动和非孢子形成的放线菌，被命名为 F435，从巴基斯坦乔利斯坦沙漠的土壤样本中分离得到。该菌株的分类地位通过多相分类学方法确定。细胞呈球菌形，单个或成对排列。新型菌株在 15-37°C（最佳温度为 25-30°C）、pH 值 7-11（最佳 pH 值为 7-8）和 0-8%（w/v）NaCl（最佳 0%）的条件下生长。基于 16S rRNA 基因序列的 Blast 分析结果表明，MON 2.2 与该菌株的相似度最高，为 97.4%，其次是 CPCC 204711（95.2%）。在系统发育树中，菌株 F435与该属的唯一成员形成了一个稳健的聚类。细胞壁中存在的肽聚糖异构体为 ll-二氨基庚二酸。主要脂肪酸为 anteiso-C。该菌株的特征性极性脂为双磷脂酰甘油、磷脂酰甘油、磷酸甘油脂和糖脂。主要的menaquinone 为 MK-9(H)。基因组 G+C 含量计算为 73.5mol%。菌株 F435 与 MON 2.2 之间的数字 DNA-DNA 杂交（GGDC）和平均核苷酸同一性（ANI）值分别为 24.6%和 81.8%。基于表型、化学分类、系统发育和系统基因组分析的结果，菌株 F435 代表了该属的一个新种，建议将其命名为 sp. nov.。其模式菌株为 F435（=JCM 33648=CGMCC 1.17443）。对该属的描述也进行了修订。
