sp. nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus .

A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435, was isolated from soil sample collected from the Cholistan Desert, Pakistan. The taxonomic position of the strain was established by using a polyphasic taxonomic approach. The cells were coccoid-shaped and found in single or arrangement of pairs. The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %). Results of blast analysis based on 16S rRNA gene sequences showed that MON 2.2 was its closest relative with 97.4 % similarity followed by CPCC 204711 (95.2 %). In phylogenetic trees, strain F435 formed a robust cluster with the only member of the genus . The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid. The major fatty acid was determined to be anteiso-C. Characteristic polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids and glycolipids. The predominant menaquinone was MK-9(H). The genomic G+C content was calculated as 73.5 mol%. The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435 and MON 2.2 were 24.6 and 81.8 %, respectively. Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435 represents a novel specie of the genus , for which the name sp. nov. is proposed. The type strain is F435 (=JCM 33648=CGMCC 1.17443). The description of the genus has also been emended.