Department of Orthopedics, Xinqiao Hospital, Army Medical University, Chongqing 400037, P.R. China.
Emergency Department of University‑Town Hospital of Chongqing Medical University, Chongqing 401331, P.R. China.
Mol Med Rep. 2019 Aug;20(2):1075-1084. doi: 10.3892/mmr.2019.10364. Epub 2019 Jun 6.
In recent years, acellular spinal cord scaffolds have been extensively studied in tissue engineering. Notably, acellular spinal cord scaffolds may be used to treat spinal cord injury; however, the method of preparation can result in low efficiency and may affect the biological properties of cells. This study aimed to use EDC crosslinking, combined with chemical extraction for tissue decellularization, in order to improve the efficiency of acellular scaffolds. To make the improved stent available for the clinical treatment of spinal cord injury, it is necessary to study its immunogenicity. Therefore, this study also focused on the adherence of rat bone marrow mesenchymal stem cells to scaffolds, and their differentiation into neuron‑like cells in the presence of suitable trophic factors. The results revealed that EDC crosslinking combined with chemical extraction methods may significantly improve the efficiency of acellular scaffolds, and may also confer better biological characteristics, including improved immunogenicity. Notably, it was able to promote adhesion of rat bone marrow mesenchymal stem cells and their differentiation into neuron‑like cells. These results suggested that the improved preparation method may be promising for the construction of multifunctional acellular scaffolds for the treatment of spinal cord injury.
近年来，脱细胞脊髓支架在组织工程中得到了广泛的研究。值得注意的是，脱细胞脊髓支架可用于治疗脊髓损伤；然而，其制备方法可能导致效率低下，并可能影响细胞的生物学特性。本研究旨在使用 EDC 交联，结合化学提取进行组织脱细胞化，以提高脱细胞支架的效率。为了使改良支架可用于临床治疗脊髓损伤，有必要研究其免疫原性。因此，本研究还重点研究了大鼠骨髓间充质干细胞在支架上的黏附及其在合适的营养因子存在下分化为神经元样细胞的情况。结果表明，EDC 交联结合化学提取方法可能显著提高脱细胞支架的效率，并且还可能赋予更好的生物学特性，包括改善免疫原性。值得注意的是，它能够促进大鼠骨髓间充质干细胞的黏附和向神经元样细胞的分化。这些结果表明，改良的制备方法可能有望用于构建用于治疗脊髓损伤的多功能脱细胞支架。
