Faculté des Sciences de la Nature et de la Vie, Université Abdelhamid Ibn Badis de Mostaganem, 27000, Mostaganem, Algeria; Laboratoire de Recherche « Santé et Production Animale », Ecole Nationale Supérieure Vétérinaire d'El-Harrach, 16000, Algiers, Algeria.
Laboratoire de Bactériologie Médicale, Institut Pasteur d'Algérie, 16000, Algiers, Algeria.
Comp Immunol Microbiol Infect Dis. 2019 Jun;64:163-167. doi: 10.1016/j.cimid.2019.03.009. Epub 2019 Mar 14.
In the last few years, antimicrobial resistant (AMR) Escherichia coli have been detected in newborn chickens suggesting their vertical transmission from breeding birds to their offspring. However, little is known about the presence of AMR E. coli in the reproductive organs of broiler breeders. The aim of this study was to investigate the presence of E. coli in the ovaries of healthy broiler breeders and to study their antimicrobial resistance. Samples from broiler breeders (n = 80) collected from 80 different broiler breeder flocks were included in this study. Antibiotic susceptibility testing was performed using disk diffusion method according to Clinical and Laboratory Standards Institute guidelines. Minimal inhibitory concentrations (MICs) of five antimicrobial agents were determined by Etest. PCR and sequencing were used to detect the bla genes. E. coli were detected in the ovaries of thirty seven out of 80 (46.25%) sampled flocks. High levels of resistance to various first-line antimicrobial agents were recorded in E. coli isolates. This study showed that 89.18% of E. coli isolates were multidrug resistant (MDR). Furthermore, MDR extended-spectrum β-lactamases (ESBL)-producing E. coli were detected in the ovaries of four different broiler breeder flocks. Molecular characterization revealed that three isolates harboured bla gene and one isolate expressed bla gene. In addition, one bla -producing E. coli co-harboured the bla gene. These findings would contribute to a better epidemiological understanding of MDR E. coli for improve existing preventive strategies in order to reduce the dissemination of antimicrobial resistance in the broiler production system.
在过去的几年中，已在新生鸡中检测到具有抗微生物耐药性（AMR）的大肠杆菌，这表明其从种禽垂直传播给后代。然而，人们对种鸡繁殖器官中存在 AMR 大肠杆菌知之甚少。本研究旨在调查健康种鸡的卵巢中是否存在大肠杆菌，并研究其对抗微生物药物的耐药性。本研究纳入了 80 个不同种鸡群的 80 只种鸡样本。采用纸片扩散法按照临床和实验室标准协会指南进行抗生素药敏试验。采用 Etest 测定五种抗菌药物的最小抑菌浓度（MIC）。通过 PCR 和测序检测 bla 基因。在 37/80（46.25%）个采集的鸡群的卵巢中检测到大肠杆菌。在大肠杆菌分离株中记录到对各种一线抗菌药物的高水平耐药性。本研究表明，89.18%的大肠杆菌分离株为多药耐药（MDR）。此外，在四个不同的种鸡群的卵巢中检测到 MDR 产extended-spectrum β-lactamases（ESBL）的大肠杆菌。分子特征表明，三个分离株携带 bla 基因，一个分离株表达 bla 基因。此外，一个产生 bla 的大肠杆菌还共同携带 bla 基因。这些发现有助于更好地了解 MDR 大肠杆菌的流行病学，从而改进现有的预防策略，以减少肉鸡生产系统中抗微生物耐药性的传播。
