Zainab L, Ibrar K, Sadiq A, Hamid A K, Ullah Midrar, Noor R
Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan.
Institute of Biological Sciences Sarhad University of Science and Information Technology, Peshawar, Pakistan.
Saudi J Biol Sci. 2022 Jun;29(6):103280. doi: 10.1016/j.sjbs.2022.103280. Epub 2022 Apr 20.
In human diet, poultry meat is an important component due to the presence of vitamins, proteins and minerals. But poultry meat can be contaminated by pathogenic bacteria which are responsible for food borne infections. The current study was therefore aimed at identification of , a common pathogen causing food borne infections, in chicken samples (n = 400) collected from three districts of KhyberPukhtunkhwa; Peshawar, Kohat and Nowshera. The isolates were identified by Gram staining, API strips and through PCR (Universal Stress Protein). A total of 174 samples were positive for among the collected chicken samples. The isolates were resistant to TE, NOR and NA while were sensitive to MEM, TZP and FOS. The results were statistically significant having value P ≤ 0.05 in ANOVA. The isolates showed different antibiotic resistance genes; OXA-1, CTX-M15, blaTEM, QnrS, TetA, AAC, AAD, sul1 and sul2 which is the molecular explanations of their antibiotic resistance pattern. The PCR products were sequenced by Next Generation Sequencing (NGS) and the results revealed mutations in AAC gene (M120T and R197T) and CTX-M15 (A85V, N122D, A148S and G247D). To prevent and treat pathogenic diseases, the use of antimicrobial agents in animal husbandry are of utmost concern. The over-use and misuse of antimicrobial agents has made pathogenic multi drugs resistant making it a causative agent for many diseases in human beings. The results of the current study may be helpful for the physicians the better management of the diseases caused by .
在人类饮食中，禽肉因含有维生素、蛋白质和矿物质而成为重要组成部分。但禽肉可能被导致食源性感染的病原菌污染。因此，本研究旨在从开伯尔-普赫图赫瓦省的白沙瓦、科哈特和瑙谢拉三个地区采集的400份鸡肉样本中鉴定一种导致食源性感染的常见病原菌。通过革兰氏染色、API试条和PCR（通用应激蛋白）对分离株进行鉴定。在所采集的鸡肉样本中，共有174份样本检测出该病原菌呈阳性。这些分离株对四环素、诺氟沙星和萘啶酸耐药，而对美罗培南、替卡西林/克拉维酸和磷霉素敏感。方差分析结果具有统计学意义，P值≤0.05。这些分离株显示出不同的抗生素耐药基因；OXA-1、CTX-M15、blaTEM、QnrS、TetA、AAC、AAD、sul1和sul2，这是它们抗生素耐药模式的分子解释。通过下一代测序（NGS）对PCR产物进行测序，结果显示AAC基因（M120T和R197T）和CTX-M15（A85V、N122D A148S和G247D）发生了突变。为预防和治疗致病性疾病，畜牧业中抗菌剂的使用至关重要。抗菌剂的过度使用和滥用已使致病性病原菌产生多重耐药性，使其成为人类许多疾病的病原体。本研究结果可能有助于医生更好地管理由该病原菌引起的疾病。
