Department of Neurology, L226, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, United States of America.
Department of Neurology, L226, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, United States of America; Research, VA Portland Health Care System, 3710 SW U.S. Veterans Hospital Road, Portland, OR 97239, United States of America.
J Neuroimmunol. 2019 Sep 15;334:576972. doi: 10.1016/j.jneuroim.2019.576972. Epub 2019 May 27.
The anti-oxidant lipoic acid (LA) is beneficial in murine models of multiple sclerosis (MS) and has recently been shown to slow brain atrophy in secondary progressive MS. The mechanism of these effects by LA is incompletely understood but may involve effects on microglia. The objective of this study is to understand how LA affects microglial cells. We cultured primary microglial cells from C57BL/6 adult mice brains and stimulated the cells with lipopolysaccharide (LPS) and interferon gamma (IFN-γ) in the presence or absence of LA. We demonstrate the inhibition of phagocytosis, rearrangement of actin, and formation of membrane blebs in stimulated microglia in the presence of LA. These experiments suggest that LA causes changes in microglial actin, which may lead to alterations in phagocytosis, mobility, and migration.
抗氧化剂硫辛酸 (LA) 有益于多发性硬化症 (MS) 的鼠模型，并且最近已被证明可减缓继发性进行性 MS 中的脑萎缩。LA 产生这些作用的机制尚不完全清楚，但可能涉及对小胶质细胞的影响。本研究的目的是了解 LA 如何影响小胶质细胞。我们从小鼠大脑的 C57BL/6 成年鼠中培养原代小胶质细胞，并在存在或不存在 LA 的情况下用脂多糖 (LPS) 和干扰素 γ (IFN-γ) 刺激细胞。我们证明在 LA 存在下刺激的小胶质细胞中吞噬作用、肌动蛋白重排和膜泡形成受到抑制。这些实验表明 LA 引起小胶质细胞肌动蛋白的变化，这可能导致吞噬作用、迁移和迁移的改变。
