长读纳米孔测序法构建一株耐碳青霉烯类铜绿假单胞菌的草图基因组
Long-read nanopore sequencing-based draft genome of a carbapenem-resistant Pseudomonas aeruginosa isolate.
机构信息
Infectious Diseases Department, Mengchao Hepatobiliary Hospital of Fujian Medical University, 312 Xihong Road, Gulou District, Fuzhou, 350025, Fujian, China.
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Fisheries College, Jimei University, 43 Yindou Road, Xiamen, 361021, Fujian, China.
出版信息
J Glob Antimicrob Resist. 2019 Dec;19:207-209. doi: 10.1016/j.jgar.2019.05.023. Epub 2019 Jun 5.
OBJECTIVES
Pseudomonas aeruginosa is a common Gram-negative bacterium causing various serious infections, such as lower respiratory tract infection and urinary tract infection in catheterised patients. Here we report the draft genome sequence of a carbapenem-resistant P. aeruginosa (CRPA) isolate.
METHODS
The genome of the CRPA isolate was sequenced using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads.
RESULTS
The resulting assembly was highly contiguous, containing a total of 6624003bp with a GC content of 66.21%. Annotation identified 6389 protein-coding genes. Mutations in the oprD and mexR genes conferred resistance to carbapenems in the CRPA isolate.
CONCLUSION
The draft genome sequence of this CRPA isolate could provide a solid basis for further research on the resistance mechanisms and the development of drug therapy for drug resistance genes.
目的
铜绿假单胞菌是一种常见的革兰氏阴性菌，可引起各种严重感染，如下呼吸道感染和留置导尿管患者的尿路感染。在此，我们报告了一株碳青霉烯类耐药铜绿假单胞菌（CRPA）分离株的基因组草图序列。
方法
使用 Illumina 短读长和 Oxford Nanopore 长读长相结合的方法对 CRPA 分离株的基因组进行测序。
结果
所得的组装序列高度连续，共包含 6624003bp，GC 含量为 66.21%。注释鉴定出 6389 个编码蛋白的基因。在 CRPA 分离株中，oprD 和 mexR 基因突变赋予了对碳青霉烯类药物的耐药性。
结论
该 CRPA 分离株的基因组草图序列可为进一步研究耐药机制和开发耐药基因的药物治疗提供坚实的基础。
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