Sodium sulfide selectively induces oxidative stress, DNA damage, and mitochondrial dysfunction and radiosensitizes glioblastoma (GBM) cells.

Glioblastoma (GBM) has a poor prognosis despite intensive treatment with surgery and chemoradiotherapy. Previous studies using dose-escalated radiotherapy have demonstrated improved survival; however, increased rates of radionecrosis have limited its use. Development of radiosensitizers could improve patient outcome. In the present study, we report the use of sodium sulfide (NaS), a hydrogen sulfide (HS) donor, to selectively kill GBM cells (T98G and U87) while sparing normal human cerebral microvascular endothelial cells (hCMEC/D3). NaS also decreased mitochondrial respiration, increased oxidative stress and induced γH2AX foci and oxidative base damage in GBM cells. Since NaS did not significantly alter T98G capacity to perform non-homologous end-joining or base excision repair, it is possible that GBM cell killing could be attributed to increased damage induction due to enhanced reactive oxygen species production. Interestingly, NaS enhanced mitochondrial respiration, produced a more reducing environment and did not induce high levels of DNA damage in hCMEC/D3. Taken together, this data suggests involvement of mitochondrial respiration in NaS toxicity in GBM cells. The fact that survival of LN-18 GBM cells lacking mitochondrial DNA (ρ) was not altered by NaS whereas the survival of LN-18 ρ cells was compromised supports this conclusion. When cells were treated with NaS and photon or proton radiation, GBM cell killing was enhanced, which opens the possibility of HS being a radiosensitizer. Therefore, this study provides the first evidence that HS donors could be used in GBM therapy to potentiate radiation-induced killing.