Crowley P J, Fischlschweiger W, Coleman S E, Bleiweis A S
Department of Oral Biology, College of Dentistry, University of Florida, Gainesville 32610.
Infect Immun. 1987 Nov;55(11):2695-700. doi: 10.1128/iai.55.11.2695-2700.1987.
Mutans streptococci (MS) representing eight different serotypes were tested for their ability to coaggregate in vitro with oral actinomyces and other streptococcal species. Of the mutans streptococci tested, only strains of S. cricetus (formerly S. mutans serotype a) displayed pronounced coaggregations and only with certain strains of actinomyces. S. cricetus coaggregated, by lactose nonreversible mechanisms, with serotype 4 Actinomyces naeslundii WVU963 and WVU924 and with serotype 2 Actinomyces odontolyticus WVU758. The first pair was disaggregated by protein denaturants (e.g., sodium dodecyl sulfate and urea) and EDTA. This coaggregation was inhibited when the streptococcal, but not the actinomyces, partner was pretreated with either heat or protease, suggesting the presence of a protein mediator on only the streptococcal cell surface. The S. cricetus-A. odontolyticus coaggregation appeared to involve protein components on each cell, as shown by the lack of coaggregation after pretreatment of either cell type with heat or proteases. This coaggregation was also reversed by sodium dodecyl sulfate and urea, as well as by sodium deoxycholate, but not by EDTA. The data indicate that different mechanisms may be involved in each of these coaggregations.
对代表八种不同血清型的变形链球菌（MS）进行了测试，以检测其在体外与口腔放线菌及其他链球菌属共同聚集的能力。在所测试的变形链球菌中，只有仓鼠链球菌（以前的变形链球菌血清型a）菌株表现出明显的共同聚集，且仅与某些放线菌菌株发生这种现象。仓鼠链球菌通过乳糖不可逆机制与血清型4的内氏放线菌WVU963和WVU924以及血清型2的溶牙放线菌WVU758发生共同聚集。第一组配对组合可被蛋白质变性剂（如十二烷基硫酸钠和尿素）及乙二胺四乙酸（EDTA）解聚。当对链球菌而非放线菌进行热预处理或蛋白酶预处理时，这种共同聚集受到抑制，这表明仅在链球菌细胞表面存在一种蛋白质介导物。仓鼠链球菌与溶牙放线菌的共同聚集似乎涉及每个细胞上的蛋白质成分，这可通过对任一种细胞类型进行热预处理或蛋白酶预处理后共同聚集消失得以证明。这种共同聚集也可被十二烷基硫酸钠、尿素以及脱氧胆酸钠逆转，但不能被EDTA逆转。数据表明，这些共同聚集中的每一种可能涉及不同的机制。
