McBride B C, Van der Hoeven J S
Infect Immun. 1981 Aug;33(2):467-72. doi: 10.1128/iai.33.2.467-472.1981.
The role of interbacterial adherence in the colonization of the rate oral cavity was investigated with aggregating and nonaggregating strains of Veillonella alcalescens and Streptococcus mutans. V. alcalescens V-1 and S. mutans M-7 rapidly formed large stable aggregates when mixed in vitro. Aggregates could be reduced in size by sonication, but they could not be completely dispersed, indicating that bonding between the organisms was strong. V. alcalescens V-1 did not coaggregate with S. mutans C67-1, and V. alcalescens OMZ193 did not coaggregate with either S. mutans strain C67-1 or M-7. Osborne-Mendel rats monoassociated with either S. mutans C67-1 or M-7 were inoculated with veillonellae, molar teeth were removed at 2 h and at 14 days, and the number of veillonellae was determined. At 2 h post-inoculation there were 600 times as many colony-forming units of V. alcalescens V-1 adherent to the teeth of animals monoassociated with S. mutans M-7 when compared with animals monoassociated with the nonaggregating S. mutans C67-1. The number of colony-forming units of V. alcalescens V-1 was 1,000 times greater than the number of nonaggregating V. alcalescens OMZ193 in S. mutans M-7-infected animals. Similar results were obtained when teeth were samples 14 days after inoculation. Veillonellae inoculated into the mouths of germfree animals rapidly disappeared from tooth surfaces.
采用产碱韦荣球菌和变形链球菌的聚集性菌株与非聚集性菌株，研究了细菌间黏附在大鼠口腔定植中的作用。产碱韦荣球菌V-1和变形链球菌M-7在体外混合时迅速形成大的稳定聚集体。聚集体经超声处理后尺寸可减小，但不能完全分散，这表明生物体之间的结合很强。产碱韦荣球菌V-1不与变形链球菌C67-1共聚集，产碱韦荣球菌OMZ193不与变形链球菌C67-1或M-7共聚集。将单定殖有变形链球菌C67-1或M-7的奥斯本-孟德尔大鼠接种韦荣球菌，在2小时和14天时拔除磨牙，测定韦荣球菌数量。接种后2小时，与单定殖非聚集性变形链球菌C67-1的动物相比，单定殖变形链球菌M-7的动物牙齿上附着的产碱韦荣球菌V-1的菌落形成单位数量多600倍。在感染变形链球菌M-7的动物中，产碱韦荣球菌V-1的菌落形成单位数量比非聚集性产碱韦荣球菌OMZ193的数量多1000倍。接种14天后采集牙齿样本时也得到了类似结果。接种到无菌动物口腔中的韦荣球菌迅速从牙齿表面消失。
