Cherif Aicha O, Person Marine De, Messaouda Mhamed Ben, Abderrabba Manef, Moussa Fathi
Université de Carthage, Laboratoire Matériaux, Molécules et Applications.
LETIAM, -Lip (Sys)², EA 7357, Paris-Sud, Université Paris-Saclay, IUT d'Orsay.
J Oleo Sci. 2019 Jul 1;68(7):637-647. doi: 10.5650/jos.ess19017. Epub 2019 Jun 10.
In the present work, a high-performance liquid chromatographic method coupled with mass spectrometry (HILIC-HPLC /ESI-MS) was used for the characterization and the quantification of glycerophospholipids (GPLs) classes and their molecular species in three genetically different Tunisian apricot cultivars (bitter, sweet and semi-sweet apricots). The application of the proposed method to the analysis of apricot oil allowed to separate and identify 74 molecular species of GPLs. Phosphatidylcholine (PC) class was found to be the most abundant GLPs in the three seed oils (38.6-62.4%) especially in bitter apricot, followed by phosphatidylinositol (PI) and phosphatidylethanolamine (PE) classes with values of 8.3-38.9% and 1.7-25.4% respectively. Phosphatidic acid (PA), phosphatidylglycerol (PG) and lysophosphatidylcholine (LPC) compounds were minor ones with maximums of 11.3%, 9.8% and 9.2% respectively. The results we obtained for the three Tunisian apricot seed varieties clearly indicate that the phospholipids of Tunisian apricot are of great interest. In fact, the high content of phosphatidylcholine (PC) determines it as a suitable and valuable source for obtaining corresponding phospholipids concentrates.
在本研究中,采用高效液相色谱-质谱联用方法(亲水作用液相色谱-高效液相色谱/电喷雾电离质谱,HILIC-HPLC /ESI-MS)对三个遗传不同的突尼斯杏品种(苦杏仁、甜杏仁和半甜杏仁)中的甘油磷脂(GPLs)类别及其分子种类进行表征和定量分析。将所提出的方法应用于杏仁油分析,能够分离并鉴定出74种甘油磷脂分子种类。结果发现,磷脂酰胆碱(PC)类别是三种杏仁油中含量最丰富的甘油磷脂(占38.6 - 62.4%),尤其是在苦杏仁中,其次是磷脂酰肌醇(PI)和磷脂酰乙醇胺(PE)类别,含量分别为8.3 - 38.9%和1.7 - 25.4%。磷脂酸(PA)、磷脂酰甘油(PG)和溶血磷脂酰胆碱(LPC)化合物含量较少,最高分别为11.3%、9.8%和9.2%。我们针对突尼斯的三个杏种所获得的结果清楚地表明,突尼斯杏仁中的磷脂具有重要价值。事实上,磷脂酰胆碱(PC)的高含量使其成为获取相应磷脂浓缩物的合适且有价值的来源。