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太平洋白虾中肌球蛋白基因的全基因组鉴定与表达谱分析

Genome-Wide Identification and Expression Profiles of Myosin Genes in the Pacific White Shrimp, .

作者信息

Zhang Xiaoxi, Yuan Jianbo, Zhang Xiaojun, Liu Chengzhang, Li Fuhua, Xiang Jianhai

机构信息

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.

出版信息

Front Physiol. 2019 May 21;10:610. doi: 10.3389/fphys.2019.00610. eCollection 2019.

Abstract

As the main structural protein of muscle fiber, myosin is essential for multiple cellular processes or functions, especially for muscle composition and development. Although the shrimp possess a well-developed muscular system, the knowledge about the myosin family in shrimp is far from understood. In this study, we performed comprehensive analysis on the myosin genes in the genome of the Pacific white shrimp, . A total of 29 myosin genes were identified, which were classified into 14 subfamilies. Among them, subfamily was significantly expanded in the penaeid shrimp genome. Most of the Myo2 subfamily genes were primarily expressed in abdominal muscle, which suggested that Myo2 subfamily genes might be responsible for the well-developed muscular system of the penaeid shrimp. hybridization detection showed that the slow-type muscle myosin gene was mainly localized in pleopod muscle and superficial ventral muscle of the shrimp. This study provides valuable insights into the evolutionary and functional characterization of myosin genes in shrimps, which provides clues for us to understand the well-developed muscular system of shrimp.

摘要

作为肌纤维的主要结构蛋白,肌球蛋白对于多种细胞过程或功能至关重要,尤其是对于肌肉组成和发育。尽管虾拥有发达的肌肉系统,但关于虾中肌球蛋白家族的知识仍远未被了解。在本研究中,我们对太平洋白虾基因组中的肌球蛋白基因进行了全面分析。共鉴定出29个肌球蛋白基因,它们被分为14个亚家族。其中, 亚家族在对虾基因组中显著扩增。大多数Myo2亚家族基因主要在腹肌中表达,这表明Myo2亚家族基因可能是对虾发达肌肉系统的原因。 杂交检测表明,慢型肌肉肌球蛋白基因主要定位在虾的腹足肌肉和腹侧浅肌中。本研究为虾中肌球蛋白基因的进化和功能特征提供了有价值的见解,为我们理解虾发达的肌肉系统提供了线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c863/6537884/32fc22c33c35/fphys-10-00610-g001.jpg

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