Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China.
Oxid Med Cell Longev. 2019 Apr 16;2019:4275984. doi: 10.1155/2019/4275984. eCollection 2019.
Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. The idea of therapeutic angiogenesis in ischemic myocardium is a promising strategy for MI patients. Buyang Huanwu decoction (BHD), a famous Chinese herbal prescription, exerted antioxidant, antiapoptotic, and anti-inflammatory effects, which contribute to cardio-/cerebral protection. Here, we aim to investigate the effects of BHD on angiogenesis through the caveolin-1 (Cav-1)/vascular endothelial growth factor (VEGF) pathway in MI model of mice.
C57BL/6 mice were randomly divided into 3 groups by the table of random number: (1) sham-operated group (sham, = 15), (2) AMI group (AMI+sham, = 20), and (3) BHD-treated group (AMI+BHD, = 20). 2,3,5-Triphenyltetrazolium chloride solution stain was used to determine myocardial infarct size. Myocardial histopathology was tested using Masson staining and hematoxylin-eosin staining. CD31 immunofluorescence staining was used to analyze the angiogenesis in the infarction border zone. Western blot analysis, immunofluorescence staining, and/or real-time quantitative reverse transcription polymerase chain reaction was applied to test the expression of Cav-1, VEGF, vascular endothelial growth factor receptor 2 (VEGFR2), and/or phosphorylated extracellular signal-regulated kinase (p-ERK). All statistical analyses were performed using the SPSS 20.0 software and GraphPad Prism 6.05. Values of < 0.05 were considered as statistically significant.
Compared with the AMI group, the BHD-treated group showed a significant improvement in the heart weight/body weight ratio, echocardiography images, cardiac function, infarct size, Mason staining of the collagen deposition area, and density of microvessel in the infarction border zone ( < 0.05). Compared with the AMI group, BHD promoted the expression of Cav-1, VEGF, VEGFR2, and p-ERK in the infarction border zone after AMI. BHD could exert cardioprotective effects on the mouse model with AMI through targeting angiogenesis via Cav-1/VEGF signaling pathway.
急性心肌梗死（AMI）仍然是全球发病率和死亡率的主要原因。在缺血心肌中进行治疗性血管生成的想法是 MI 患者的一种有前途的策略。补阳还五汤（BHD）是一种著名的中药方剂，具有抗氧化、抗凋亡和抗炎作用，有助于心脏/大脑保护。在这里，我们旨在通过 Cav-1/血管内皮生长因子（VEGF）通路研究 BHD 对 MI 模型中小鼠血管生成的影响。
C57BL/6 小鼠通过随机数表随机分为 3 组：（1）假手术组（sham，n=15）、（2）AMI 组（AMI+sham，n=20）和（3）BHD 治疗组（AMI+BHD，n=20）。2,3,5-三苯基氯化四氮唑溶液染色用于确定心肌梗死面积。采用 Masson 染色和苏木精-伊红染色检测心肌组织病理学变化。CD31 免疫荧光染色用于分析梗死边界区的血管生成。Western blot 分析、免疫荧光染色和/或实时定量逆转录聚合酶链反应用于检测 Cav-1、VEGF、血管内皮生长因子受体 2（VEGFR2）和/或磷酸化细胞外信号调节激酶（p-ERK）的表达。所有统计分析均使用 SPSS 20.0 软件和 GraphPad Prism 6.05 进行。 < 0.05 被认为具有统计学意义。
与 AMI 组相比，BHD 治疗组的心脏重量/体重比、超声心动图图像、心功能、梗死面积、胶原沉积区 Mason 染色和梗死边界区微血管密度均有显著改善（ < 0.05）。与 AMI 组相比，BHD 促进了 AMI 后梗死边界区 Cav-1、VEGF、VEGFR2 和 p-ERK 的表达。BHD 可能通过靶向 Cav-1/VEGF 信号通路促进血管生成，对 AMI 小鼠模型发挥心脏保护作用。
