Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Biosens Bioelectron. 2018 Jul 1;110:23-37. doi: 10.1016/j.bios.2018.03.037. Epub 2018 Mar 19.
Vascular endothelial growth factor (VEGF) is a key regulator of vascular formation and a predominant protein biomarker in cancer angiogenesis. Owing to its crucial roles in the cancer metastasis, VEGF detection and quantification is of great importance in clinical diagnostics. Today, there exist a wide variety of detection strategies for identifying many types of disease biomarkers, especially for VEGF. As artificial single-stranded DNA or RNA oligonucleotides with catalytic and receptor properties, aptamers have drawn lots of attention to be applied in biosensing platforms due to their target-induced conformational changes as well as high stability and target versatility. So far, various sensitivity-enhancement techniques in combination with a broad range of smart nanomaterials have integrated into the design of novel aptasensors to improve detection limit and sensitivity of analyte detection. This review article provides a brief classification and description of the research progresses of aptamer-based biosensors and nanobiosensors for the detection and quantitative determination of VEGF based on optical and electrochemical platforms.
血管内皮生长因子（VEGF）是血管形成的关键调节剂，也是癌症血管生成的主要蛋白生物标志物。由于其在癌症转移中的关键作用，VEGF 的检测和定量在临床诊断中非常重要。目前，存在多种用于识别多种类型疾病生物标志物的检测策略，特别是针对 VEGF。由于人工单链 DNA 或 RNA 寡核苷酸具有催化和受体特性，适体因其靶标诱导的构象变化以及高稳定性和靶标多功能性而引起了广泛关注，可应用于生物传感平台。到目前为止，各种灵敏度增强技术与广泛的智能纳米材料相结合，已融入新型适体传感器的设计中，以提高分析物检测的检测限和灵敏度。本文简要介绍了基于适体的生物传感器和纳米生物传感器在光学和电化学平台上基于光学和电化学平台对 VEGF 的检测和定量测定的研究进展，并对其进行了分类和描述。
