铁皮石斛 B-BOX 基因的比较分析及其在不同处理下的表达模式分析。
Comparative analysis of B-BOX genes and their expression pattern analysis under various treatments in Dendrobium officinale.
机构信息
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China.
School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
出版信息
BMC Plant Biol. 2019 Jun 10;19(1):245. doi: 10.1186/s12870-019-1851-6.
BACKGROUND
Studies have demonstrated that BBX (B-BOX) genes play crucial roles in regulatory networks controlling plant growth, developmental processes and stress response. Nevertheless, comprehensive study of BBX genes in orchids (Orchidaceae) is not well studied. The newly released genome sequences of Dendrobium officinale and Phalaenopsis equestris have allowed a systematic analysis of these important BBX genes in orchids.
RESULTS
Here we identified 19 (DoBBX01-19) and 16 (PeBBX01-16) BBX genes from D. officinale and P. equestris, respectively, and clustered into five clades (I-V) according to phylogenetic analysis. Thirteen orthologous, two DoBBXs paralogous and two PeBBXs paralogous gene pairs were validated. This gene family mainly underwent purifying selection, but five domains experienced positive selection during evolution. Noteworthy, the expression patterns of root, root_tips, stem, leaf, speal, column, lip, and flower_buds revealed that they might contribution to the formation of these tissues. According to the cis-regulatory elements analysis of BBX genes, qRT-PCR experiments were carried out using D. officinale PLBs (protocorm-like bodies) and displayed that these BBX genes were differentially regulated under AgNO, MeJA (Methyl Jasmonate), ABA (abscisic acid) and SA (salicylic acid) treatments.
CONCLUSIONS
Our analysis exposed that DoBBX genes play significant roles in plant growth and development, and response to different environmental stress conditions of D. officinale, which provide aid in the selection of appropriate candidate genes for further functional characterization of BBX genes in plants.
背景
研究表明，B-BOX（BBX）基因在调控植物生长、发育过程和应激反应的网络中发挥着关键作用。然而，兰花（Orchidaceae）中 BBX 基因的综合研究还不够深入。铁皮石斛和蝴蝶兰基因组序列的最新发布，使得对这些重要的 BBX 基因在兰花中的系统分析成为可能。
结果
我们从铁皮石斛和蝴蝶兰中分别鉴定了 19 个（DoBBX01-19）和 16 个（PeBBX01-16）BBX 基因，并根据系统发育分析将其聚类为五个分支（I-V）。验证了 13 个同源基因、2 个 DoBBX 基因的旁系同源物和 2 个 PeBBX 基因的旁系同源物。该基因家族主要经历了纯化选择，但在进化过程中，有 5 个结构域经历了正选择。值得注意的是，根、根尖、茎、叶、穗、柱、唇和花蕾的表达模式表明，它们可能对这些组织的形成有贡献。根据 BBX 基因的顺式调控元件分析，对铁皮石斛 PLBs（原球茎样体）进行了 qRT-PCR 实验，结果显示这些 BBX 基因在 AgNO、MeJA（茉莉酸甲酯）、ABA（脱落酸）和 SA（水杨酸）处理下表现出差异表达。
结论
我们的分析表明，DoBBX 基因在铁皮石斛的生长发育和对不同环境胁迫条件的响应中发挥着重要作用，这为进一步研究 BBX 基因在植物中的功能提供了有价值的候选基因。
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