Cao Yunpeng, Han Yahui, Meng Dandan, Abdullah Muhammad, Yu Jiangliu, Li Dahui, Jin Qing, Lin Yi, Cai Yongping
School of Life Sciences, Anhui Agricultural University, Hefei, 230036, China.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036, China.
Funct Integr Genomics. 2018 Nov;18(6):673-684. doi: 10.1007/s10142-018-0620-1. Epub 2018 Jun 13.
GDSL-type esterase/lipase (GELP) is mainly characterized by a conserved GDSL domain at N terminus, and is widely found in all living species, both prokaryotes and eukaryotes. GELP gene family consists of a wide range of members playing important roles in plant physiological processes, such as development, stress responses, and functional divergences. In our study, 597 GELP genes were identified from six Rosaceae genomes (i.e., Fragaria vesca, Prunus persica, Prunus avium, Prunus mume, Pyrus bretschneideri, and Malus domestica) by a comprehensive analysis. All GELP genes were further divided into ten subfamilies based on phylogenetic tree analysis. Subfamily D and subfamily E are the two largest subfamilies. Microcollinearity analysis suggested that WGD/segmental events contribute to the expansion of the GELP gene family in M. domestica and P. bretschneideri compared to F. vesca, P. persica, P. avium, and P. mume. Some PbGELPs were expressed during the fruit development of P. bretschneideri and pollen tubes, indicating their activity in these tissues. The expression divergence of PbGELP duplication gene pairs suggests that many mutations were allowed during evolution, although the structure of GELP genes was highly conserved. The current study results provided the feasibility to understand the expansion and evolution patterns of GELP in Rosaceae genomes, and highlight the function during P. bretschneideri fruits and pollen tubes development.
GDSL 型酯酶/脂肪酶（GELP）主要特征是在 N 端有一个保守的 GDSL 结构域，广泛存在于原核生物和真核生物等所有生物物种中。GELP 基因家族由众多成员组成，它们在植物生理过程中发挥重要作用，如发育、应激反应和功能分化。在我们的研究中，通过综合分析从六个蔷薇科基因组（即野草莓、桃、甜樱桃、梅、白梨和苹果）中鉴定出 597 个 GELP 基因。基于系统发育树分析，所有 GELP 基因进一步分为十个亚家族。亚家族 D 和亚家族 E 是两个最大的亚家族。微共线性分析表明，与野草莓、桃、甜樱桃和梅相比，全基因组复制/片段重复事件促进了苹果和白梨中 GELP 基因家族的扩张。一些白梨 GELP 基因在白梨果实发育和花粉管中表达，表明它们在这些组织中有活性。白梨 GELP 重复基因对的表达差异表明，尽管 GELP 基因结构高度保守，但在进化过程中允许发生许多突变。当前的研究结果为了解蔷薇科基因组中 GELP 的扩张和进化模式提供了可行性，并突出了其在白梨果实和花粉管发育过程中的功能。
