Ranganathan S, Davis D G, Hood R D
Department of Biology, University of Alabama, Tuscaloosa 35487.
Teratology. 1987 Aug;36(1):45-9. doi: 10.1002/tera.1420360107.
Ethanol was tested for teratogenicity in Drosophila melanogaster. Treatment consisted of rearing the fly larvae in media containing initial ethanol concentrations of 0%, 4%, 8%, or 14% by weight. Emerging flies were inspected for gross malformations. A low frequency of malformations was seen among controls (0.82%), increasing to 10.36% of emerging adults at the highest ethanol dose. The most common malformation involved the legs (segments missing or distorted or complete absence) and wings (uninflated, distorted, or absent). Less frequent defects included fused or missing mouth parts and missing halteres. Also, by exposing staged larvae to ethanol and examining the emerging flies, developmental stage sensitivity of Drosophila was investigated in terms of timing of treatment initiation. The results suggested that the incidence of defects increased with length of exposure. These results support the assumption that ethanol itself is the causative agent in ethanol-induced developmental toxicity and further support the use of Drosophila for developmental toxicity screening.
在黑腹果蝇中对乙醇的致畸性进行了测试。处理方法是将果蝇幼虫饲养在初始乙醇浓度按重量计分别为0%、4%、8%或14%的培养基中。对羽化出的果蝇进行外观畸形检查。在对照组中观察到低频率的畸形（0.82%），在最高乙醇剂量下，羽化出的成虫畸形率增至10.36%。最常见的畸形涉及腿部（节段缺失、扭曲或完全缺失）和翅膀（未充气、扭曲或缺失）。不太常见的缺陷包括口器融合或缺失以及平衡棒缺失。此外，通过将不同发育阶段的幼虫暴露于乙醇并检查羽化出的果蝇，根据处理开始的时间研究了果蝇的发育阶段敏感性。结果表明，缺陷的发生率随暴露时间的延长而增加。这些结果支持了乙醇本身是乙醇诱导发育毒性的致病因素这一假设，并进一步支持将果蝇用于发育毒性筛选。
