State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
PLA Rocket Army Characteristic Medical Center, Beijing, China.
Vector Borne Zoonotic Dis. 2019 Dec;19(12):933-944. doi: 10.1089/vbz.2019.2453. Epub 2019 Jun 10.
The Japanese encephalitis virus (JEV), a mosquito-borne zoonotic pathogen, is the major cause of viral encephalitis worldwide. An investigation of mosquito species diversity, JEV infection rate, and seasonal population fluctuations of in Huaihua County, Hunan Province, China, revealed the distribution of vector mosquito populations and genotypes and molecular characteristics of current, common JEV strains in this region. Research on mosquito species diversity in different habitats in Huaihua revealed that local community composition was relatively simple, including five species from four genera (two spp., one sp., one sp., and one sp.). was clearly the dominant species comprising 94.2-98.6% of all specimens and was always the most common species captured in paddy fields, pigpens, and human dwellings. The seasonal abundance of was relatively even, with a single seasonal peak in late August. Two Huaihua JEV strains isolated from the mosquito and pig were highly congruent. The genetic affinities were determined by analyzing capsid/premembrane (C/PrM) and envelope (E) gene variation. The results showed that they were of genotype III and most closely related to the live, attenuated vaccine strains SAl4-14-2 and SA14 and JaGAr01. The Huaihua E protein shares high similarity (mosquito 98.8% and pig 97.6%) at the nucleotide level with the SA14-14-2 vaccine. Although we found that the E gene sequences of the Huaihua JEV mosquito strain and pig strain have 11 and 15 amino acid site substitutions compared with the SAl4-14-2 vaccine, key sites that associated with JEV's antigenic activity and neurovirulence were unchanged. The SA14-14-2 vaccine should therefore be effective in preventing JEV infection in the Huaihua region.
日本脑炎病毒（JEV）是一种经蚊传播的人畜共患病病原体，是全球病毒性脑炎的主要病因。在中国湖南省怀化市，我们调查了蚊种多样性、JEV 感染率以及的季节性种群波动，揭示了该地区病媒蚊种群的分布以及当前常见 JEV 株的基因分型和分子特征。对怀化市不同生境中蚊种多样性的研究表明，当地的群落组成相对简单，包括四个属的五个种（两种 spp.，一种 sp.，一种 sp. 和一种 sp.）。 显然是优势种，占所有标本的 94.2-98.6%，并且总是在稻田、猪圈和民居中捕获的最常见的物种。 的季节性丰度相对均匀，只有一个 8 月底的单峰。从蚊子和猪中分离出的两种怀化 JEV 株高度一致。通过分析衣壳/前膜（C/PrM）和包膜（E）基因变异来确定遗传亲和力。结果表明，它们属于基因型 III，与活减毒疫苗株 SAl4-14-2 和 SA14 以及 JaGAr01 最为密切相关。怀化 E 蛋白在核苷酸水平上与 SA14-14-2 疫苗具有很高的相似性（蚊子 98.8%，猪 97.6%）。尽管我们发现怀化 JEV 蚊子株和猪株的 E 基因序列与 SAl4-14-2 疫苗相比有 11 个和 15 个氨基酸位点取代，但与 JEV 抗原活性和神经毒力相关的关键位点没有改变。因此，SA14-14-2 疫苗应该能有效预防怀化市 JEV 感染。
