Department of Chemistry, Vaal University of Technology, Private Bag X021 Vanderbijlpark, 1900, South Africa.
Department of Chemistry, Vaal University of Technology, Private Bag X021 Vanderbijlpark, 1900, South Africa.
Anal Biochem. 2019 Sep 1;580:36-41. doi: 10.1016/j.ab.2019.06.008. Epub 2019 Jun 8.
Nanoparticles in biomedicine, requires a detailed understanding of the biocompatibility and toxicity of nanoparticles in human beings. The biological and physicochemical properties of nanoparticles bring new challenges with regards to management of potential adverse health effects following exposure. This work reports on the synthetic optimization of condition of copper sulphide nanoparticles and the cytotoxicity effects of the particles on the human cervical carcinoma cells. CuS nanoparticles synthesized produced a single and different XRD phases in basic and acidic media, respectively. Temperature variation influenced both the optical and morphological properties of the nanoparticles. The highest temperature (95 °C) resulted in particles with superior properties compared to the ones synthesized at lower temperatures. The cytotoxicity tests showed that the particles were nontoxic at low to moderate concentrations and only induced toxicity at higher concentrations. Particles synthesized at 95 °C were less toxic compared to other nanoparticles as determined by their CC values.
生物医药中的纳米粒子，需要详细了解纳米粒子在人类中的生物相容性和毒性。纳米粒子的生物学和物理化学性质给潜在的不良健康影响的管理带来了新的挑战。本工作报告了硫化铜纳米粒子的合成优化条件，以及这些粒子对人宫颈癌细胞的细胞毒性作用。在碱性和酸性介质中分别合成了具有单一和不同 XRD 相的 CuS 纳米粒子。温度变化影响了纳米粒子的光学和形态特性。最高温度（95°C）导致的粒子具有优于在较低温度下合成的粒子的性能。细胞毒性试验表明，这些粒子在低浓度到中等浓度时没有毒性，只有在高浓度时才会引起毒性。通过 CC 值测定，95°C 下合成的粒子比其他纳米粒子的毒性更小。
