Rehana Dilaveez, Mahendiran D, Kumar R Senthil, Rahiman A Kalilur
Post-Graduate and Research Department of Chemistry, The New College (Autonomous), Chennai 600 014, India; Department of Chemistry, Justice Basheer Ahmed Sayeed College for Women (Autonomous), Chennai 600 018, India.
Post-Graduate and Research Department of Chemistry, The New College (Autonomous), Chennai 600 014, India.
Biomed Pharmacother. 2017 May;89:1067-1077. doi: 10.1016/j.biopha.2017.02.101. Epub 2017 Mar 11.
Copper oxide (CuO) nanoparticles were synthesized by green chemistry approach using different plant extracts obtained from the leaves of Azadirachta indica, Hibiscus rosa-sinensis, Murraya koenigii, Moringa oleifera and Tamarindus indica. In order to compare their efficiency, the same copper oxide nanoparticles was also synthesized by chemical method. Phytochemical screening of the leaf extracts showed the presence of carbohydrates, flavonoids, glycosides, phenolic compounds, saponins, tannins, proteins and amino acids. FT IR spectra confirmed the possible biomolecules responsible for the formation of copper oxide nanoparticles. The surface plasmon resonance absorption band at 220-235nm in the UV-vis spectra also supports the formation of copper oxide nanoparticles. XRD patterns revealed the monoclinic phase of the synthesized copper oxide nanoparticles. The average size, shape and the crystalline nature of the nanoparticles were determined by SEM, TEM and SAED analysis. EDX analysis confirmed the presence of elements in the synthesized nanoparticles. The antioxidant activity was evaluated by three different free radical scavenging assays. The cytotoxicity of copper oxide nanoparticles was evaluated against four cancer cell lines such as human breast (MCF-7), cervical (HeLa), epithelioma (Hep-2) and lung (A549), and one normal human dermal fibroblast (NHDF) cell line. The morphological changes were evaluated using Hoechst 33258 staining assay. Copper oxide nanoparticles synthesized by green method exhibited high antioxidant and cytotoxicity than that synthesized by chemical method.
采用绿色化学方法，利用从印楝、扶桑、九里香、辣木和罗望子的叶子中提取的不同植物提取物合成了氧化铜（CuO）纳米颗粒。为了比较它们的效率，还通过化学方法合成了相同的氧化铜纳米颗粒。对叶提取物进行的植物化学筛选表明，其中存在碳水化合物、黄酮类化合物、糖苷、酚类化合物、皂苷、单宁、蛋白质和氨基酸。傅里叶变换红外光谱（FT IR）证实了可能负责氧化铜纳米颗粒形成的生物分子。紫外可见光谱中220 - 235nm处的表面等离子体共振吸收带也支持氧化铜纳米颗粒的形成。X射线衍射（XRD）图谱揭示了合成的氧化铜纳米颗粒的单斜相。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）和选区电子衍射（SAED）分析确定了纳米颗粒的平均尺寸、形状和晶体性质。能量散射X射线（EDX）分析证实了合成纳米颗粒中元素的存在。通过三种不同的自由基清除试验评估了抗氧化活性。评估了氧化铜纳米颗粒对四种癌细胞系（如人乳腺癌（MCF - 7）、宫颈癌（HeLa）、上皮瘤（Hep - 2）和肺癌（A549））以及一种正常人皮肤成纤维细胞（NHDF）细胞系的细胞毒性。使用Hoechst 33258染色试验评估了形态变化。绿色方法合成的氧化铜纳米颗粒比化学方法合成的表现出更高的抗氧化和细胞毒性。
