通过反相高效液相色谱法分析作为其丹磺酰衍生物的多胺。
Analysis of polyamines as their dabsyl derivatives by reversed-phase high-performance liquid chromatography.
作者信息
Koski P, Helander I M, Sarvas M, Vaara M
机构信息
National Public Health Institute, Helsinki, Finland.
出版信息
Anal Biochem. 1987 Jul;164(1):261-6. doi: 10.1016/0003-2697(87)90395-2.
The polyamines putrescine, cadaverine, spermidine, and spermine and the corresponding mono-N-acetylpolyamines can be separated as their dimethylaminoazobenzenesulfonyl derivatives in a single analysis in less than 22 min. The method employs reversed-phase high-performance liquid chromatography (Spherisorb S5 ODS2 column) with an acetonitrile/acetate buffer gradient elution system and detection in the visible (436 nm) region. The detection limit for a single dimethylaminoazobenzenesulfonylpolyamine is less than 2 pmol.
多胺腐胺、尸胺、亚精胺和精胺以及相应的单 -N- 乙酰多胺，可在不到 22 分钟的单次分析中，作为其二甲基氨基偶氮苯磺酰衍生物进行分离。该方法采用反相高效液相色谱法（Spherisorb S5 ODS2 柱），以乙腈 / 醋酸盐缓冲液梯度洗脱系统，并在可见光（436 nm）区域进行检测。单个二甲基氨基偶氮苯磺酰多胺的检测限小于 2 pmol。
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